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Fig. 3. Xtrb2 expression during early Xenopus embryogenesis. (A, B) In situ hybridization of Xtrb2 at stage 15. Anterior dorsal view (A) and lateral view (B) are shown. A white arrow indicates the dorsal midline. (C, D) Xtrb2 expression at stage 22/23. Anterior view of the head (C) and lateral view (D). Strong expression was observed in the optic placode, otic vesicle, and neural crest. Arrowheads indicate a streak of cells from the otic vesicle (ov) towards the cement gland (CG). Weak staining of somites was also detected. (E–I) Expression pattern of Xtrb2 at stage 26. Lateral view in the head (E), dorsal view (F), and transverse sections (G–I) as indicated in (E) and (F). Note the lack of Xtrb2 expression in the floorplate (fp) along the neural tube. Weak expression was observed in pronephros. Abbreviations: cg, cement gland; mn, motor neuron; nc, neural crest; pl, placodes; n, notochord; fp, floor plate; pn, pronephros; op, optic vesicle; ov, otic vesicle; ab, anterior branchial; pb, posterior branchial; h, hyoid neural crest. |