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Figure 1. Expression of trim2 during neurogenesis. (a–i) trim2 expression was assessed by whole-mount in situ hybridization (Wmish) at different developmental stages. (a,b) Maternal trim2 transcripts were detected in the animal (an) opposed to the vegetal (vg) hemisphere of Xenopus embryos. (c) NF stage 17, anterior view, trim2 transcripts were evident in the eye field (ef) and in the forming neural tube (nt). (d–f) NF stage 21, lateral, anterior and dorsal views. trim2 transcripts were detected in eye vesicle (ev), neural tube (nt), olfactory (ol), and otic placode (op), as well as cement gland anlage (cg). (g–i) At NF stage 25, trim2 expression became more distinct in eye vesicle (eb), forebrain (fb), midbrain (mb), olfactory placode (ol), otic placode (op), and neural tube (nt). (j–l) NF stage 33, trim2 in fore- (fb), mid- (mb), and hindbrain (hb), eye (ey), olfactory pit (olp), otic vesicle (ov), spinal cord (sc), and trigeminal ganglion (tg). Scales as indicated. (strd-mo n = 20 embryos; trim2-mo n = 16 embryos (E); p = 1.97E-09, unpaired χ2 test, χ2 = 36, df =1, phenotype occurrence (po) =16, p < 0.001). |
