| |
Figure 2. Trim2/trim2 are expressed in differentiated neurons of the forming central nervous system at the tailbud stage. (A) (a–c) Expression of trim2 mRNA at NF stage 35, anterior, ventral, and lateral views, trim2 transcripts were detected in the forming brain (br), olfactory pit (olp), spinal cord (sc), and the ventral blood island (vbi). (d–h) Twenty-micrometer gelatine/albumin sections at the level of fore-, mid-, hindbrain, and spinal cord. trim2 is expressed in the prosencephalon (pc), olfactory pit, ganglion cell layer (gc), lens epithelium (ls), hypothalamus (hy), motor neurons (mn), otic vesicle (ov), and spinal cord (sc). Faint expression was observed in the ventral blood island. (i–k) Sagittal sections reveal trim2 expression in the ganglion cell layer, lens epithelium, brain, spinal cord, notochord (nc), somites (so), and ventral blood island. (B) Whole-mount immunochemistry. (a–c) At NF stage 35 (anterior, dorsal, and lateral views), Trim2 protein expression overlaps strongly with trim2 mRNA. (d–i) Twenty-micrometer gelatine/albumin transverse sections along the fore-, mid-, hindbrain, and spinal cord region. Trim2 protein was detected in the developing CNS, cement gland, dorsal root entry zone (drez), ganglion cells, hindbrain, hypothalamus, ion-secreting cells (isc), motor neurons, olfactory pit, otic vesicle, prosencephalon (pc), prethalamus (pth), presumptive radial glial cells (rg), somites (so), and ventral blood island. |
