| |
FIG. 2. (A) Distribution of X-translin in stage VI and maturing Xenopus oocytes. Western blot analysis with a-X-translin antibodies were
developed in the germinal vesicle (VG) or the cytoplasm (Cy) of stage VI (VI) or maturing oocytes at 1, 2, 3, and 4 h after progesterone addition
(1 h, 2 h, 3 h, 4 h, respectively). Equivalent protein amounts corresponding to one germinal vesicle or one cytoplasm were loaded per lane.
(B) Subcellular localisation of X-translin in XTC cells. Single a-X-translin immunofluorescence in interphase cell (1, X-translin) and
pro-metaphase cell (2, X-translin) as visualised by propidium iodide staining (2, DNA) and double a-X-translin/a-b-tubulin immunofluorescence
(3, X-translin, 3, b-tubulin, respectively) in a metaphase cell. The bar in the lower right micrograph corresponds to 50 mm (same
magnification for all micrographs). The localisation of X-translin in the different stages of the cell cycle was identical for all the cells observed
in all the immunostainings. |
