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ttyh3xenopus forebrain 

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Experiment details for ttyh3

Characterization of tweety gene (ttyh1-3) expression in Xenopus laevis during embryonic development.

Characterization of tweety gene (ttyh1-3) expression in Xenopus laevis during embryonic development.

Gene Clone Species Stages Anatomy
ttyh3.S laevis NF stage 19 to NF stage 20 forebrain
ttyh3.S laevis NF stage 22 to NF stage 23 forebrain
ttyh3.S laevis NF stage 25 to NF stage 26 forebrain
ttyh3.S laevis NF stage 25 to NF stage 26 forebrain
ttyh3.S laevis NF stage 29 and 30 to NF stage 31 forebrain
ttyh3.S laevis NF stage 33 and 34 to NF stage 35 and 36 forebrain

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  Fig. 1. Whole mount in situ hybridization showing spatial and temporal expression of tweety homologs in developing X. laevis embryos. Row 1 and 2: dorsal views. Rows 3, 4, 5: both lateral (top embryo, with dorsal side on top) and dorsal (bottom embryo) views. Anterior is oriented to the right for all embryos. Row 6: close up of anterior part of the embryo, with a lateral view (dorsal side on top) and a dorsal view (with anterior directed upwards). (A, G, M) Stages 19–20 (late neurula). (B, H, N) Stages 22–23 (early tailbud). (C, I, O) Stages 25–26 (mid-tailbud). (D, J, P) Stages 29–31 (late tailbud). (E, K, Q) Stages 34–35 (swimming tadpole). (F, L, R) Stages 34–35 (swimming tadpole, close up). (A–F) Ttyh1 expression. (G–L) Ttyh2 expression. (M–R) Ttyh3 expression. Arrowheads indicate regions of expression. (fb, forebrain; e, eye; hb, hindbrain; mb, midbrain; ot, otic vesicle; pa, pharyngeal arches; sc, spinal cord; nt, neural tube; so, somites; Roman numerals: cranial ganglia.) Scale bars = 1.0 mm.

Gene Clone Species Stages Anatomy
ttyh3.S laevis NF stage 25 to NF stage 26 forebrain

  ttyh3 (tweety family member 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25-26, dorsal view, anterior right.

Gene Clone Species Stages Anatomy
ttyh3.S laevis NF stage 33 and 34 to NF stage 35 and 36 forebrain

  ttyh3 ((tweety family member 3) ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 34 & 35, lateral view, anterior right, dorsal up, (above) , and dorsal view (below).

Gene Clone Species Stages Anatomy
ttyh3.S laevis NF stage 35 and 36 to NF stage 37 and 38 forebrain

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  Fig. 2. Histological analysis of tweety homolog expression in X. laevis stages 35–37 (swimming tadpole) using whole-mount in situ hybridization. Transverse 18 μm sec- tions along the anterior–posterior axis with dorsal to the top. (A–O) 10× magnification images focused on neural regions with signal. (A′–O′) 4× magnification images to show to the absence of signal in other regions. (A, F, K) Sections showing forebrain. (B, G, L) Sections showing midbrain. (C, H, M) Sections showing hindbrain. (D, I, N) Sections showing anterior spinal cord. (E, J, O) Sections showing posterior spinal cord. (A–E) Ttyh1 expression. (F–J) Ttyh2 expression. (K–O) Ttyh3 expression. Arrowheads indicate regions of expression. (asc, anterior spinal cord; mb, midbrain; hb, hindbrain; fb, forebrain; e, eye; nc, neural crest; ot, otic vesicle; psc, posterior spinal cord; sc, spinal cord; Roman numerals: cranial ganglia.) Scale bars = 0.25 mm.