|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ttyh2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
roof plate
,
cranial ganglion
,
eye
,
posterior neural tube
,
anterior neural tube
,
[+]
|
ttyh2
|
|
laevis
|
NF stage 29 and 30
|
roof plate
,
posterior neural tube
,
anterior neural tube
,
neural tube
|
ttyh1
|
|
laevis
|
NF stage 19
to
NF stage 20
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
eye primordium
,
[+]
|
ttyh1
|
|
laevis
|
NF stage 22
to
NF stage 23
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
ttyh1
|
|
laevis
|
NF stage 25
to
NF stage 26
|
otic vesicle
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
ttyh1
|
|
laevis
|
NF stage 29 and 30
to
NF stage 31
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
ventricular zone
,
[+]
|
ttyh1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
retina
,
brain
,
forebrain
,
midbrain
,
[+]
|
ttyh3
|
|
laevis
|
NF stage 19
to
NF stage 20
|
brain
,
forebrain
,
somite
,
eye primordium
,
posterior neural tube
,
[+]
|
ttyh3
|
|
laevis
|
NF stage 25
to
NF stage 26
|
brain
,
forebrain
,
hindbrain
,
spinal cord
,
eye
,
[+]
|
|
|
Fig. 1. Whole mount in situ hybridization showing spatial and temporal expression of tweety homologs in developing X. laevis embryos. Row 1 and 2: dorsal views. Rows 3, 4, 5: both lateral (top embryo, with dorsal side on top) and dorsal (bottom embryo) views. Anterior is oriented to the right for all embryos. Row 6: close up of anterior part of the embryo, with a lateral view (dorsal side on top) and a dorsal view (with anterior directed upwards). (A, G, M) Stages 19–20 (late neurula). (B, H, N) Stages 22–23 (early tailbud). (C, I, O) Stages 25–26 (mid-tailbud). (D, J, P) Stages 29–31 (late tailbud). (E, K, Q) Stages 34–35 (swimming tadpole). (F, L, R) Stages 34–35 (swimming tadpole, close up). (A–F) Ttyh1 expression. (G–L) Ttyh2 expression. (M–R) Ttyh3 expression. Arrowheads indicate regions of expression. (fb, forebrain; e, eye; hb, hindbrain; mb, midbrain; ot, otic vesicle; pa, pharyngeal arches; sc, spinal cord; nt, neural tube; so, somites; Roman numerals: cranial ganglia.) Scale bars = 1.0 mm. |
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ttyh3 (tweety family member 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25-26, dorsal view, anterior right. |
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ttyh3 (tweety family, member 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 34 & 35, lateral view, anterior right, dorsal up, (left) , and dorsal view (right). |
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ttyh3 ((tweety family member 3) ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 34 & 35, lateral view, anterior right, dorsal up, (above) , and dorsal view (below). |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ttyh2
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
retina
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
ttyh1
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
otic vesicle
,
retina
,
brain
,
forebrain
,
midbrain
,
[+]
|
|
|
Fig. 2. Histological analysis of tweety homolog expression in X. laevis stages 35–37 (swimming tadpole) using whole-mount in situ hybridization. Transverse 18 μm sec- tions along the anterior–posterior axis with dorsal to the top. (A–O) 10× magnification images focused on neural regions with signal. (A′–O′) 4× magnification images to show to the absence of signal in other regions. (A, F, K) Sections showing forebrain. (B, G, L) Sections showing midbrain. (C, H, M) Sections showing hindbrain. (D, I, N) Sections showing anterior spinal cord. (E, J, O) Sections showing posterior spinal cord. (A–E) Ttyh1 expression. (F–J) Ttyh2 expression. (K–O) Ttyh3 expression. Arrowheads indicate regions of expression. (asc, anterior spinal cord; mb, midbrain; hb, hindbrain; fb, forebrain; e, eye; nc, neural crest; ot, otic vesicle; psc, posterior spinal cord; sc, spinal cord; Roman numerals: cranial ganglia.) Scale bars = 0.25 mm. |