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Fig. 2. Histological analysis of tweety homolog expression in X. laevis stages 35–37 (swimming tadpole) using whole-mount in situ hybridization. Transverse 18 μm sec- tions along the anterior–posterior axis with dorsal to the top. (A–O) 10× magnification images focused on neural regions with signal. (A′–O′) 4× magnification images to show to the absence of signal in other regions. (A, F, K) Sections showing forebrain. (B, G, L) Sections showing midbrain. (C, H, M) Sections showing hindbrain. (D, I, N) Sections showing anterior spinal cord. (E, J, O) Sections showing posterior spinal cord. (A–E) Ttyh1 expression. (F–J) Ttyh2 expression. (K–O) Ttyh3 expression. Arrowheads indicate regions of expression. (asc, anterior spinal cord; mb, midbrain; hb, hindbrain; fb, forebrain; e, eye; nc, neural crest; ot, otic vesicle; psc, posterior spinal cord; sc, spinal cord; Roman numerals: cranial ganglia.) Scale bars = 0.25 mm. |
