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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
cdh3
|
|
laevis
|
NF stage 17
|
ectoderm
,
notochord
,
somite
,
neural plate
,
paraxial mesoderm
|
folr1
|
|
laevis
|
NF stage 15
|
ectoderm
,
notochord
,
presomitic mesoderm
,
neural plate
,
paraxial mesoderm
|
folr1
|
|
laevis
|
NF stage 17
|
ectoderm
,
mesoderm
,
notochord
,
somite
,
neural plate
|
|
|
Fig. 1.
Folate receptor 1 expression during Xenopus laevis neural tube formation. (A) Time course of Xenopus laevis neurulation. hpf, hours post-fertilization. (B) Western blot assays from homogenates of wild-type (WT) or Xenopus laevis folate receptor 1 (Folr1)-overexpressing embryos at the indicated developmental stages. (C) Folate is present during Xenopus neural tube formation and folate levels do not change upon Folr1 knockdown. Data show folate levels measured by ELISA assay in homogenates from stage 13-17 (14.5-19 hpf) WT, folate receptor 1 morpholino 1 (Folr1-MO1)- or standard control morpholino (CMO)-injected embryos. Mean±s.e.m.; n=8 independent measurements from n>40 embryos/group; average embryo weight: 2±0.4 mg. (D,E) Neural plate stage embryos were processed for Folr1 (green) and β-tubulin (D, red) or C-cadherin (E, red) immunostaining. Shown are representative transverse sections of immunostained samples at neural plate stages 15 (D) and 17 (E). (D) Arrow in top panel indicates Folr1 localization at the apical neural plate surface and arrowheads indicate localization in extracellular spaces. Bottom panels show higher magnifications of the boxed area in the panel above and illustrate Folr1 localization to extracellular spaces. L, notochord lumen; Not, notochord. (E) Arrow indicates that Folr1 localizes to the apical neural plate surface along with adherens junction protein C-cadherin. Bottom panels show higher magnifications of the boxed area in the panel above. Scale bars: 20 μm. |
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Display additional annotations [+]
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Fig. S3. Knockdown of folate receptor 1 in mesoderm or non-neural ectoderm does
not induce neural tube defects. Targeted blastomeres of 16-cell-stage embryos were
unilaterally microinjected with 3 pmol/cell morpholino against the Xenopus laevis Folr1
(Folr1-MO) along with Alexa 594-dextran conjugate in indicated blastomeres (left).
Neural plate stage embryos were then sectioned and processed for immunostaining with
anti-β-tubulin (grayscale) and Sox2 (green). Red indicates MO-containing cells. Scale
bars: 20 μm. |