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Fig. 2 Embryonic expression of ube2d3.2. (now called ube2d4) (A) A polyclonal antibody raised to amino acids 1–147 of ube2d3.2 was used to probe control embryos (uninjected) and embryos expressing FLAG-tagged ube2d3.2(-FLAG). (B) Equal quantities of total embryonic protein
from the indicated stages were loaded onto an SDS-PAGE gel and probed with the polyclonal antibody raised against ube2d3.2,
upper panel. A duplicate membrane was probed with pre-immune
serum from the rabbit used to produce the specific ube2d3.2 antibody, middle panel (both the upper and middle panels were exposed for the same time). Tubulin was used as a loading control lower panel. (
C) Expression pattern
of ube2d3.2 mRNA at stages
28 and 35. Panels show lateral
views with anterior to left and the
right panel is a magnification of
the middle panel. AS, antisense
probe. The somites (s), eye (e), otic
vesicle (o), branchial arches (ba),
and pronephric primordium (p)
are indicated. |
