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FIGURE 5 Staining of neuromuscular junctions of mouse, chicken, and Xenopus. Frozen sections through junctional regions of
mouse diaphragm (a, d, and g), chicken posterior latissimus dorsi (b, e, and h), and Xenopus sartorius (c, f, and i) muscles were
stained with antibodies. Methods were as described in Fig. 1, except that fluorescein-avidin was used only in antifilamin staining
of mouse muscle and staining of chicken muscle used lO-fold lower concentrations of antivinculin and ninefold lower
concentrations of fGAR. R-a-BuTx stained regions (not shown) in all cases coincided with the brightly staining, membraneassociated
structures pictured here. The antibodies used were antivinculin, (a-c); anti-a-actinin, (d-f); antifilamin, (g-i). The
results show that, except for anti-c~-actinin reaction with Xenopus muscle (f), junctional regions of all three species are stained
by the antibodies. Some weak extrajunctional staining is also apparent in a and e; staining of the contractile apparatus is evident
in f, and, more faintly, in d. Bars, 20 pm. The bar in g also applies to a and d, and the bar in i applies to the rest of the panels. |
