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Fig. 2. In situ hybridization analysis of Wnt11-R expression during Xenopus development. (A) Dorsal view of st. 13 embryo showing earliest Wnt11-R expression in the dorsal region of the developing somites (arrow). (B) Dorsal view at st. 22 showing transcripts in the somite and neural tube. (C) Transverse section at st. 22 shows that Wnt11-R transcripts are limited to the dorsal margin of the somite (arrow) and the dorsal neural tube. (D) Dorsal view at st. 26 showing Wnt11-R expression in the somites, branchial arches, neural tube, and brain. (E) Lateral view of st. 26 embryo. (F) Ventral view of st. 26 embryo. (G) Lateral view at st. 28 shows Wnt11-R in the heart myocardium (arrow). (H) Lateral view at st. 28 with expression of MHCα marking the precardiac region. (I) Ventral view of st. 28 embryo showing Wnt11-R expression in precardiac tissue. (J) Ventral view of st. 28 embryo stained for MHCα expression. (K) Lateral view at st. 35 showing Wnt11-R expression in the branchial arches, somites, neural tube, fin, and the heart. (L) Transverse section through the a medial region of a st. 35 embryo showing Wnt11-R transcripts in the dorsal margin of the somite, dorsal neural tube, and mesenchymal cells within the fin. (M–O) Transverse sections progressing from anterior to posterior regions through the heart tube of a st. 35 embryo showing Wnt11-R expression in the myocardium of the heart. Sections show the future outflow tract and ventricle (M), the ventricle (N), and the atria (O). (P) MHCα expression in section equivalent to that in O, showing expression throughout myocardial layer. (Q) Wnt11-R is expressed throughout the myocardium of the st. 45 heart, although expression is lower in atrial tissues. Atria and ventricular (vent) tissues are indicated. (R) MHCα expression in the st. 45 heart showing strong expression throughout the myocardium. |
