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Figure 2. In situ hybridization analysis of Wnt2 expression during Xenopus embryonic development. A: Lateral view of stage 20 embryo showing Wnt2 expression in the lateral region just posterior to the head. B: Lateral view of stage 23 embryo showing increased extent of Wnt2 expression domain. C: Transverse section through Wnt2 expressing region of a stage 26 embryo showing expression restricted to deep mesoderm tissue adjacent to foregut endoderm. D: Lateral view of stage 28 embryo. The domain of Wnt2 expression is greatest at this stage and extends ventrally toward the heart forming region. E: Transverse section of stage 28 embryo showing Wnt2 expression restricted to mesoderm. F: Lateral view of stage 32 embryo. Residual Wnt2 expression is limited to a small region of the flank (arrowhead) posterior to the pharyngeal arches. Genbank accession number for Xenopus tropicalis Wnt2 is EF447424. |