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Figure 5. xWnt6 protein is localized within the ectoderm and epithelial tissues and within the developing heart, eye, and pronephros. A-C,E-G,I-K,M-O,Q-S,U-W: Immunofluorescence staining performed on cryosectioned embryos using an anti-Wnt6 antibody. D,H,L,P,T,X: Control sections stained without primary antibody and with secondary antibody alone. Y,Z,AA,BB,CC,DD,EE,FF: Higher magnification immunofluorescence staining performed on cryosectioned embryos using an anti-Wnt6 antibody (green) and an anti-troponin T antibody (red). GG,HH: Confocal optical frontal sections showing Wnt6 (green) and troponin T staining (red) in isolated Xenopus hearts. II,JJ: Immunofluorescence staining performed on cryosectioned embryos using an anti-Wnt6 antibody (green) and an anti-rhodopsin antibody (red). KK,LL,MM: Confocal three-dimensional images showing Wnt6 staining of the proximal tubules. The location of the sections is indicated for each developmental stage. Numerical figures indicate developmental stage. a, atrium; ba, branchial arches; ce, corneal epithelium; e, eye; ec, ectoderm, ee, endocardial endothelium; en, endocardium; ev, eye vesicle, f, fin; fav, future arterial valve; fg, foregut; g, gut; hs, head somite; i, intestine; l, lens; ld, liver diverculum; m, myocardium; mg, midgut; n, neuroceol; ose, ocular surface ectoderm; p, pericardium; pa, pronephric anlage; ph, pharynx; pt, pronephric tubules; r, rods; ros, rods outer segment; s, somite; vp, velar plate. Scale bars = 200 mu m in A-X, 100 mu m in Y-MM. |
