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Fig. 6. Overexpression of Evi1-hGR in the pronephros with DEX addition from stage 18 specifically suppresses the expression of proximal but not distal markers. (A,B, E–L, O–P) Lateral views and (C, D, M, N and Q, R) sections through the pronephros of tailbud or early tadpole stage embryos coinjected with Evi1-hGR mRNA and β-galactosidase mRNA and stained with the indicated markers. Control and injected sides marked with Red-gal are shown. Note the reduction of the expression of the X-Delta-1 (A–D), EphA4 (E,F) and XSMP-30 (G, H) proximal tubule and xWT1 (K–N) and nephrin (O, R) glomus markers (arrowheads) while the expression of the Gremlin (G, H) and xClC-K (I, J) distal tubule and duct markers is unaffected (arrow). Abbreviations: pn, pronephros; spm, splanchnic layer of the mesoderm. |
