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Fig. S1. Systemic distribution of Yap1 protein at the tailbud and tadpole stages.
A. Cartoon of the Xenopus tadpole tail, which is composed of various types of tissues. B-G. Whole-mount immunofluorescent staining at the tailbud stage (B, D, F) and early tadpole stage (C, E, G). B, C. Negative control without primary antibody. D, E. Immunostaining for Yap1. Yap1 protein was distributed throughout the whole body. F, G. Immunostaining for phosphorylated Yap1 (pYap1). The anti-pYap1 antibody recognizes the phosphorylated serine in a conserved motif of the Yap1 protein. This phosphorylation sequesters Yap1 from the nucleus and inactivates the transcription of Yap1’s target genes. B, D, F. st38 embryos. C, E, G. st46 tadpoles. Scale bar = 1 mm.
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