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Figure 1
The spatial expression pattern of Zic3 in Xenopus embryos. Series of embryo were hybridized with digoxigenin-labeled antisense Zic3 RNA. (A) Stage 10.25. (B) Stage 10.5. (C) The expression of Zic3 in dorsal lip (arrow) and prospective neural plate (arrowheads) was confirmed in the cross section of the same embryo in B. (D) Stage 12. (E) Stage 14. Asterisk indicates prospective region of mesencephalon and rhombencephalon. (F) Stage 16. White and black arrowheads indicate the lateral edge of neural plate and neural crest, respectively. (G and H) Stage 20. (I) Stage 30. In H and I, large black arrowhead, small black arrowhead, arrow, and white arrowhead indicate the telencephalon, diencephalon, mesencephalon, and rhombencephalon, respectively. Small arrow in I indicates the staining in a part of lateral mesoderm of tail. (J) Transverse section through the head region of same embryo as in I. Staining in the gut is nonspecific one, because it was detected also by the sense strand probe(data not shown). A, B, and I are the lateral views. The upper side of the panel is the animal side in A and B and the dorsal side in I. D are the dorsal views, the anterior side is toward left. H is the anterior view. The upper side is dorsal. b, blastopore; e, eye; nt, neural tube; fg, fore-gut. |