| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| znf462 | xenopus | somite [+] |
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Experiment details for znf462
Laurent A et al. (2009) Assay
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| Fig. 2. ZFPIP is maternally and zygotically expressed during Xenopus laevis development. RT-PCR experiments using Xenopus laevis embryo RNA extracts show that ZFPIP mRNAs are present in stage 1 and then decrease until stage 12. Transcription of mRNAs is enhanced at stage 17 (A). The ZFPIP protein is maternally present in oocytes. The amount of protein is then diluted during the cleavage phase. The α-PCNA antibody is used as an internal quantitative control (B). Whole mount immunohistochemistry assays are performed on Xenopus laevis embryos using an α-ZFPIP or preimmune serum at stages 5, 8, 20 and 39. The pictures show that ZFPIP is mostly present in the animal pole of blastula and progressively localises in developing neural tissues (C). Western blots confirm that the protein is preferentially localised in the animal pole. AP, VP and E correspond respectively to animal pole, vegetal pole and embryo protein extracts (D). Nuclear localization of endogenous ZFPIP or hZFPIP-FLAG protein is observed in embryos or in hZFPIP mRNA injected embryos. The immunohistochemistry is performed with α-ZFPIP or α-FLAG antibodies on Xenopus laevis embryos explants (stage 20) (E). |