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Fig. 3. Shh, Gli1, Ptc2 and Zyxin expression in CNS of the Xenopus laevis embryo A�H. In situ hybridization with probes to Shh, Gli1, Ptc2 and Zyxin on successive sections of two embryos, at stage 15 (A, C, E and G) and stage 27 (B, D, F and H). I and I′. The midneurula (stage 15) transgenic embryo expressing Zyxin-EGFP under the control of CMV promoter. Zyxin-EGFP signal is concentrated (arrowheads) along apical membranes of the flour plate cells. Dorsal view, anterior to the top. J and K. At the mid (stage 14) and late (stage 18) neurula stage transgenic embryos, Zyxin-EGFP signal is concentrated (arrowheads) along membranes of the floor plate cells. Fp�floor plae, lnp�lateral neural plate, ml and vl�mantle and ventricle layers of the neural tube respectively, hy�hypochord, np�neural plate, not�notochord, s�somite. Scale bar everywhere is 40 μm. |
