Xenbase Clone: Summary for nf2-856 [species: Xenopus laevis]

Summary

XB-CLONE-2148539

Clone Name: nf2-856	Gene: nf2.S	Species: Xenopus laevis
Description: EXRC Clone Number 856. Xenopus laevis neurofibromin 2 (merlin). Image EST 5156434, Accession BC077822. Full length. To generate probe for in situ.

Sequence:

Full length sequence: BC077822 [+]
GAGCCGTCCAGTTTGGCGGTATGGGTGTGTGTTGCTACAGTAGTATACAGAAAGGTGCTTCAAGCCACTGCACGACGATGCCGTTTCCAGGGGTATATAC
CTTGCTCCATTTGCAGTGAAAATCAGAAGTGGATTTATGAGTCTATGTGAACCAAGGAATCGCTGTCTCATCCTAATGTTCACTGTTTTTTCCCATTAGG
TGATACCCACACCCTCCCCCACGCCATAACAAGCTATACAAACATTGCCCTAAATATCCTATCAAAATTCTACGATTAGAGGTTGTCTTTTACAACTCAT
TCACATATCACTGTTTCTACCAGGAAGGAGCTGTCTTTTCAACTGGCTGCTGGCAAATGTTGGCAAACCAGGGCCAAAAGTGAACCCTACGCGTTTGCTC
AATCATCACAAAGACTTCTAAATATGGAGGATGTACCTCATACAGTATTGTGAAAAACCAAGACAACTTGCTGAAGGGGAGCCCTGCATGGCCTGGGATT
GTCAACCAGTTTCAGCTTTTGTCTTGATCAGATAGTTGGTGTTTCATTTATCCAGTGTAAAATGGCAGAGGCTATCACTTCAAGGATGAGCTTCACCTCT
CTGAAGAGGAAACAGCCAAAGACTTTCACTGTCAGAATCATTACAATGGACGCCGAGATGGAATTTAACTGTGAGATGAAGTGGAAAGGGAAGGACTTGT
TTGACTTGGTATGTCGTACTCTGGGTTTGCGGGAAACCTGGTTCTTTGGACTTCAGTATACGATAAAGGACACCATAGCATGGTTAAAGATGGATAAAAA
GGTACTGGACCATGATGTTCCAAAAGAAGAACCTGTCACCTTTCATTTTTTAGCCAAGTTCTACCCGGAAAATGTTGAGGAAGAGCTAGTACAGGATATA
ACTCAGCATCTCTTCTTCCTTCAGGTAAAGAAGCAAATTTTAGAAGAGAAAATCTACTGTCCCCCAGAAGCTTCGGTGCTTTTAGCATCATATGCCGTTC
AAGCAAAGTATGGTGATTATGATCCCTCTGTGCATAAACGTGGTTTCCTGGCAGAAGAGGAGTTACTTCCAAAAAGGGTTATAAACCTGTATCAGATGAC
ACCTGAAATGTGGGAAGAGAGAATCACTGCTTGGTATGCTGAGCATCGTGGCCGTACCAGAGATGAGGCTGAGATGGAGTACCTGAAAATTGCACAAGAC
ACGGAAATGTATGGCGTCAATTATTTTCTAATTCGGAACAAGAAAGGTACTGAGCTCTTGCTTGGTGTGGATGCTCTAGGACTTCACATTTATGACCTGG
AAAACAGACTGACTCCTAAAACCTCTTTTCCATGGAATGAGATACGTAACATCTCTTACAGTGACAAAGAGTTCACAATCAAACCCCTTGAAAAGAAGAT
AGATGTGTTCAAATTTAATTCTTCCAAGCTACGCGTCAACAAACTGATCCTGCAACTCTGCATTGGGAACCATGATTTATTCATGCGTAGGAGGAAGGCA
GACTCTTTAGAAGTACAACAGATGAAGGCTCAAGCTCGGGAGGAGAAAGCAAGGAAACAGATGGAACGCCAACGACTGGCACGGGAAAAACAGCTAAGGG
AAGAGGCTGAGCGAACACGGGATGAGTTAGAAAGAAGGCTGCTGCAGCTGAAAGACGAGGCTCAAATGGCCAATGATGCATTGATGCGGTCAGAGGAGAC
TGCAGACCTACTGGCAGAAAAAGCTCAGATCACAGAAGAGGAGGCCAAACTACTGGCCCAGAAAGCTGCAGAGGCTGAACAAGAAATGCAAAGAATTAAA
GTCACTGCCATTCGCAACGAGGAGGAAAAACGGTTAATGGAGCAAAAGGTCCTGGAGACAGAAATGCTGGCATTAAAGATGGCAGAGGAATCCGAACGGA
GAGTTAAAGAAGCTGAGCAACTGAAGCAAGACCTACAAGAGTCTCGGGATTCAGAGCGTCGAGCCAAGCAAAAGCTTCTGGAGATATCTAGTAAAACATC
TTATATGCAAGCAGCAGGGACCAGCAGCAGCAGCAGCACTGTGCCACCCACAGATGTGCCAAGTTTCAGCATGCTCAATGATAGCCTACCCTATGATTTC
AAGGACACGGACATGAAGCGTCTATCCATGGAAATTGAAAAGGAGAAGGTTGAGTACATGGAGAAGAGTCGCCACCTACAGGTACAGCTGAATGAGCTCA
AGACAGAAATCGAGTCCTTAAAACTGAAGGAAAGAGAGAGTGCCATGGATATTATGCATGAGAATGCAGGCAGCAAACAGAACACAATTAAAAAGGCAAG
GAGAGCTGTCTGCATCTGAGCGTGATCCATAGTGTGTGCCAGCTCACACTACAAAGCACACAGTGCAGAGTGGCTTTCTTTGAAGAACTCTAAGACGTCA
GCAGGGTGCTCCAGACAGCTGTAGCCTCCCCAAGGACATAAGGATTCTCAAGCAATGCTTCACAATTAGAGGAGGTCATTATTCAGTGCTACCATTTATC
TTTGCGCTGGGGACAAGGGGCATCAAAATACGTCTCCATTCACTTCTAACGTGATATGCAAAACTGCTGCTTCCCTTCTTTAATGTAAGCTAATTAAGGG
ATAGTGCTTTGTGATTCAGTCTGCTTCAACTGGGAATAGTATTTTCAAAACTATTCAAGAAATTCTATTTTAATTTGTTCAAAAGGAGATTTTTTGCTGG
AAAAATGCATTATGTTTAATACACCAATACTGTTTTTATGTATAAGAACGATAAATATTTTCCTTTGGCTATACTTTTGGTCCTTTATGCCATTTTGTTT
GTGTTTGGACTATGACACGCTTCATGACTTGCCATGGGCAATGTCTTCCAAACAGTGATACTACAGTTAAGTTTTAAAAAAAACAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAA

5' EST: CA793102 [+]
gtccagtttggcggtatgggtgtgtgttgctacagtagtatacagaaaggtgcttcaagccactgcacgacgatgccgtttccaggggtatataccttgc
tccatttgcagtgaaaatcagaagtggatttatgagtctatgtgaaccaaggaatcgctgtctcatcctaatgttcactgttttttcccattaggtgata
cccacaccctcccccacgccataacaagctatacaaacattgccctaaatatcctatcaaaattctacgattagaggttgtcttttacaactcattcaca
tatcactgtttctaccaggaaggagctgtcttttcaactggctgctggcaaatgttggcaaaccagggccaaaagtgaaccctacgcgtttgctcaatca
tcacaaagacttctaaatatggaggatgtacctcatacagtattgtgaaaaaccaagacaacttgctgaaggggagccctgcatggcctgggattgtcaa
ccagtttcagcttttgtcttgatcagatagttggtgtttcatttatccagtgtaaaatggcagaggctatcacttcaaggatgagcttcacctctctgaa
gaggaaacagccaaagactttcactgtcagaatcattacaatggacgccgagatggaatttaactgtgagatgaagtggaaagggaaggacttgtttgac
ttggtatgtcgtactctgggtttgcgggaaacctggttctttggacttcagtatacgataaaggacaccatagcatggttaagatggattaaaaggtact
gggacatgatgttccaaaagaagaactgtcacctttcattttttagccaagttctacccggaaaatgtttgaggagagctagtacaggatataactcagc
atctcttcttcttcaggta

Library:

Name: NICHD_XGC_Emb1

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.55 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 10
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: NotI 3' Restriction Site: SalI

Usage:

Whole mount in situ hybridization:

Probes:

Type	Size	Promoter	Linearization Site	Description
antisense		T7

Sequence:

Library:

Usage:

Suppliers:

Search for clone at: EXRC