XB-CLONE-1607634
Clone Name: nr2f5-806
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Gene:
nr2f5.S
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Species: Xenopus laevis
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Description: EXRC Clone Number 806.
Nuclear receptor subfamily 2, group F, member 1.
Image EST 8317884.
To generate probe for in situ.
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Sequence:
5' EST: DY564840 [+]
ccgcaatcagtgccagtactgtcgcctgaagaagtgtctcaaagttggcatgagaagagaagtgcagcgcggacggatgtctcatccacaaaccagcccg
ggccagtatactctcaacaacgttgacccctacaatgggcattcatacctaacgggattcatctccttgcttctccgagcagaaccatacccgacctctc
ggtacggggcacagtgcctccagccgaacaacatcatgggcattgagaacatctgcgagttggcggcccgccttctctttagtgccattgagtgggccaa
gaacattcctttcttccctgacttccagctctctgaccaagtgtccctgctccgtatgacatggagtgagctgtttgttcttaatgcagcgcagtgctcc
atgcccctccatgtggccccactgttggcagccgctggcctccatgcatcaccgatgtccgctgaccgggtggtggcctttatggaccatattcgaatct
ttcaggagcaggtggaaaagctgaaggcacttcatgttgactctgcagaatattcctgtttgaaagccatagctttgtttacacctgatgcggtgggact
atcagacattggccacgtggaaagcatccaagagaaatcccagtgtgccctggaagagtacgtccgaaaccagtacccaaaccaaccaacacggtttggg
aggcttttgctcc
Library:
Description: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4kb resulted in an average insert size of 1.8kb. This is a normalized library (primary library is NICHD_XGC_Emb9) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
SmaI |
EcoRI can also be used |
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Suppliers:
Search for clone at:
EXRC