Summary Attributions

Clone Name: prmt1-a-NP241	Gene: prmt1.S	Species: Xenopus laevis
Description: EXRC Clone Number NP-241. Xenopus laevis protein arginine methyltransferase 1. To generate probe for in situ.

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Sequence:

5' EST: AI031386 [+]

agtgaatggatgggatattgtctattctatgaatcaatgcttaacactgttatctatgcacgtgataaatggctgactccagatggattaatatttccag
atcgtgctacactgtatataacagctattgaggatagacaatacaaagactacaagattcactggtgggaaaatgtttatggctttgatatgtcttgcat
taaggatgtagctatcaaggagcctctggttgatgttgttgaccccaagcaactggtctcaaacgcttgccttataaaggaagttgatatttatactgtc
aaagtggatgacttgagttttacctctccattctgcctgcaagtgaaacgaaatgactacatccatgccctggttgcatatt

3' EST: AI031387 [+]

cactgttcactttttaatttcattatgaatgcaaccatataaaaaccataagttatgaaacagtcacaatgggaagctttgaaggatccatgaaacaaat
aaagggcctttccataccaagatctgagaaacttacaaaaacagtagaaaacaccaagccaagtgagtaacccatttgttcctcaataggatggatgtct
gctccacagaagggatagacggcaatattggtgaaaccagcaactgccaacaagacaattcccaaccaaataagaaaaaaaatcctgcaaaaatagtgaa
aaaacgggaatgcacaatttggagcttaattttgctgcc

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Library:

Name: Xenopus neurula plasmid library

External Dbs: Unigene laevis cDNA library

Description: Xenopus laevis polyA+ RNA embryonal stage 13 were used as source material, and directionally cloned into pBSKS+. For antisense production, plasmid should be cut using SalI, and RNA should be synthesized using T7 RNA polymerase.

Anatomy: whole organism	Stage: NF stage 13
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pBluescript KS+ Type: phagemid 5' Restriction Site: SalI 3' Restriction Site: NotI

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Usage:

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 SalI

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Suppliers:

Search for clone at: EXRC