Summary Attributions

Clone Name: rps3a/gdf3-HAR69	Gene: gdf3 , rps3a	Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-69. TGas141f11. Xenopus tropicalis growth differentiation factor 3. Not full length. To generate probe for in situ.

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Sequence:

Full length sequence: CR855767 [+]

CCCGGGGGGAGCAACTGACCCTAGGCCAACTGGAAATTAAGTTCAAGCACAACTCGTATTATGGACAACAGTTCCATCTGCGCCTCTACCGCACCCTTCA
GCTATCTCTAAAGGGTATGAGAGAGAGCAAGATGAACAGGAAGCTTCTAGTGTCACAGTCTTTCCGGCTTCTTCATAAGTCCCTCTATTTTAACCTGACC
AAGGTGGCAAAGGACTGGAAAACCCCTGAGAAGAATATGGGACTGTTATTGGAGATATATGCTAGCAGTAAACTTGCAGGAGACAACAGATCATTTGCAG
TGTGTGAACCAATCCAGTCTTTCATTTACACTTCTCTGCTCACTGTGTCTCTAGACCCATCCAGCTGCAAAACTCCGCGAGCCAAGAGAAGTACTCATTC
ATCGCCTCCAACCCCAAGTAATATCTGCAAAAAGAGGCGCCTGTACATTGACTTCAAGGATGTTGGATGGCAGAACTGGGTGATTGCACCCCGTGGTTAC
ATGGCCAACTACTGCTATGGAGAGTGCCCCTATCCACTGACAGAAATGCTCAGGGGCACAAATCATGCTGTTTTGCAGACTCTGGTGCATTCTGTAGAAC
CAGAAAGCACCCCTTTGCCTTGCTGTGCCCCCACTAAGCTGTCTCCTATCTCCATGCTATATTATGACAACAATGACAATGTGGTACTGAGGCACTATGA
AGATATGGTAGTAGATGAATGTGGTTGCAAGTGAGTTTGGCTAGGACTACTCTTCTCATTAAACTTATTGTCTAAGGGACTGCTGGCAACTTAATCATGG
AGTGATTCTCCTTGTGTGACAGTGGCTTTGACCATTAGTGTTCAGCTACAATTCCCCCCATGAAGCAAGATAGGCTTCTGTTAGCTAGAAATTGTAACAT
TCGTACCAGGAAATGTATTCCCATAAAAACAGAGACTATATTTTTTGTAAAACCTGTTTCTCCCCAATAACATTAATATGCACAAATTTTATGCATTGTT
TTTAGCTAAAATTCAGATTCTTTGGCATATGCTAGGCACAGGAAGCAAAACAAAAGTTGCCCTTTAGGTGGATGGGCCACTTTCATTTGTGACTTTTAAC
AGAGCTACAAGATGAAATGCTTTGCAAAATGACTACTGCTCTCAATTTTAGCAAAAAACAATGCATAAAATTCAGATTCTTTGGCATATGCTAGGCACAG
GAAGCAAAACAAAAGTTGCCCTTTAGGTGGATGGGCCACTTTCATTTGTGACTTTTAACAGAGCTACAAGATGAAATGCTTTGCAAAATGACTACTGCTC
TCAATTTCTTTATGTATGTATATATTGGGCATATGGCTAGGTTGGTGCCCAAGAACTGGCCTGCACTTGTTGCTTTGCTCTTGAACTGTTAAGACTAATG
TTTAAATACAGTTGATTGTTGTAAATAAAATGTGATTTAACATTAAAAAAAAAAAAAAAAAA

5' EST: AL966390 [+]

gagagcaagatgaacaggaagcttctagtgtcacagtcttttccggctttcttcataagtcccctctatttttaacctgaccaaggtggcaaaaggactt
ggaaaacccctgagaagaatatgggactgttattggagatatatgctagcagtaaacttgcangagacaacagatcatttgcagtgtgtgaaccaatcca
gtctttcatttacacttctctgctcactgtgtctctagaccatccagctgcaaactccgcgagcaagagaatactcattctcgcctccacccaagtatat
ctgcaaaagaagcgcctgtacttgacttcaggatgttggatggcagaactgggtga

3' EST: BX764956 [+]

gcttttttttttttttttttttttttttttttttaacaatgaagttgttttatttccacattaaaaaaaaataaaataggatccgttcccaccaggggga
gcaggatataaatatgtgccaaaaatgtgcagccgttccccgtgccgctccatgtgtcgcccgtcgctgggtgctgggtactcggggggggtacaataca
aagtcatatgattgggggacaaagtggggacactgaaagcccaaatccggtgccaaagcaaaacgatccccccggtc

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Library:

Name: XGC-gastrula

External Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organism	Stage: NF stage 10.5 to NF stage 12
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

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Usage:

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 EcoRI

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Suppliers:

Search for clone at: EXRC