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Summary Attributions
XB-CLONE-1652162

Clone Name: nkx2-3-902 Gene: nkx2-3.S Species: Xenopus laevis  
Description: EXRC Clone Number 902. NK2 transcription factor related, locus 3 Image EST 7979170. To generate probe for in situ

Sequence:

5' EST: DR721995 [+]

Library:

Name: NICHD_XGC_Emb10External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4kb resulted in an average insert size of 1.8kb. This is a normalized library (primary library is NICHD_XGC_Emb9) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.

Anatomy: whole organismStage: NF stage 17 to NF stage 19
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pExpress-1

Type: plasmid

5' Restriction Site: EcoRV

3' Restriction Site: NotI		Expression Image

Usage:

Whole mount in situ hybridization: Expressed in Heart.  
 
Probes:
Type Size Promoter Linearization Site Description
antisense T7 SmaI EcoRI can also be used.

Suppliers:

Search for clone at: EXRC