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Summary Expression Attributions
XB-CLONE-1820234

Clone Name: IMAGE:7204180 Gene: cfd.L Species: Xenopus laevis  
Description: EXRC Clone Number 761. Complement factor D (adipsin). To Generate Probe for In Situ.

Sequence:

5' EST: CK797755 [+]

Unigene: Xl.23957


Library:

Name: NICHD_XGC_Te2NExternal Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: RNA obtained from 6 adult male testes. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.15 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NICHD_XGC_Te2) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.

Anatomy: testisStage: frog
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pExpress-1

Type: plasmid

5' Restriction Site: EcoRV

3' Restriction Site: NotI
Expression Image

Usage:

Whole mount in situ hybridization: Expressed on Endothelial cells and blood vessels.  
 
Probes:
Type Size Promoter Linearization Site Description
antisense T7 EcoRI EcoRV and SmaI can also be used.


Suppliers:

Search for clone at: EXRC

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version: [4.5.0]

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