XB-CLONE-1967440
Sequence:5' EST: BJ067634 |
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| Name: NIBB Mochii normalized Xenopus tailbud library | External Dbs: Unigene laevis cDNA library |
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).
| Anatomy: whole organism | Stage: NF stage 25 |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map | Name: pBluescript SK- Type: phagemid 5' Restriction Site: EcoRI 3' Restriction Site: XhoI |  |
Usage:
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Search for clone at: open biosystems geneservice/RZPD imagenes