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Summary Attributions
XB-CLONE-2209955

Clone Name: IMAGE:5162533 Gene: smad10.S Species: Xenopus laevis  
 

Sequence:

5' EST: BQ398313 [+]

3' EST: BQ398312 [+]

Library:

Name: Soares NXEGExternal Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: 1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

Anatomy: eggStage: unfertilized egg stage
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pT7T3D-PacI

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: NotI
Expression Image

Usage:


Suppliers:

Search for clone at: Open Biosystems