XB-CLONE-2248176
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Clone Name: runx1-774
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Gene:
runx1.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 774. Xenopus laevis runt-related transcription factor 1 (runx1), mRNA. IMAGE:4963637. To generate probe for in situ.
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Sequence:
Full length sequence: BC057739 
[+]
GTGCAAGACTTGTTTGGGCCTCATAAACAACCACAGAACCACAAGCTGAGCACAGACACTCGACAGTGGATCCGGGATATTGGTCAGCAGAAAGGGGCGA
ATCATTTTCGTTGACTGGAAATAAAAAAACCACAAGACTTTCATACATTGCTGATTTTTTAAAAAAAAACACTAAAACCCCAGGCAGGAAGTTGAAGGAA
ACGCAATGGCCTCACATAGTGCCTTCCAGTCATTTCCTTTATACCCGCCGTGCTTCTTCAGAGATACCAGCACCAGCCGCCGCTTCACGCCGCCCTCCAC
CACTCTGAGTCCGGGGAAGATGAGCGAACCGATACCGCTGAATATCGCGGACAGCAGCGCCGCCCTGGTGGGCAAACTGAGAAGCACAGACCGGAATATG
GTGGAAGTATTGTCCGATCATCCCGGAGAACTGGTCCGCACAGACAGCCCCAACTTTCTCTGCTCGGTGCTTCCCACACACTGGAGGTGCAACAAGACCT
TGCCCATCGCTTTCAAGGTGGTGGCCTTGGGAGAAGTTCCAGATGGGACACTGGTGACAGTGATGGCTGGCAATGATGAGAACTATTCTGCTGAATTAAG
AAATGCCACAGCAGCCATGAAGAGTCAAGTGGCCCGCTTCAATGACCTGAGATTTGTCGGAAGATCTGGAAGAGGAAAAAGTTTCACCCTGACTATCACT
GTCTTCACCAATCCCCCGCAGGTGGCTACCTACCATCGCGCCATTAAAATCACAGTGGATGGACCTCGCGAGCCACGGCGACACCGACAGAAATTAGATG
AACAGACAAAACCGGGAAACTTGTCCTTTTCCGAACGACTCAGTGAACTGGAACATTTTCGCCGAACGGCCATGAGGGTCAGCCCCCATCACCCAAATCC
CATGCCCAACCCACGTGCCACCCTAAATCATTCCGCAGCTTTTAATCCTCAGCCTCAGGGTCAGATACAAGTTGCAGATACAAGACAAGTCCAGGCGTCT
CCTCCTTGGTCGTATGATCAGTCCTACCAGTACCTGGGATCCATAGCAACTCAGTCTGTACACCCTGCTACCCCCATATCTCCAGGAAGGGCAAGCAGTA
TGACGTCTCTCAGCGCTGAGCTCTCCAGCCGACTTTCAGGAGCCTCGGACCTGACAGCGTTCAGTGATCCTCGAGTTGGCATTGACCGACAGTTCTCCAC
TCTTCCTTCCATCTCTGATCCACGTATGCACTACCCAGGGGCTTTTACCTACACCCCAACCCCGGTCACTTCAGGTATAGGAATCGGCATGTCAGCAATG
ACCTCTGCTACAAGGTACCACACCTACCTACCCCCTCCATATCCTGGATCTTCCCAGGCACAAAGTAATCCCTTTCAGACCAGCTCTCCATCTTACCATC
TCTATTATGGAACATCCGCTGGATCCTACCACCAGTTCTCTATGATGTCAGGAGGAGAACGATCTCCGCCTCGCATCCTCCCACCTTGCACAAACGCTTC
CACGGGGTCCACTCTCTTGAACCCCAACCTTCCAAACCAAAGTGATGTAGTGGAGGCTGAAGGAAGCCATAGCAACTCTCCTACCAACATGGGAAGCACC
CCAAGGCTTGAGGAAGCCGTATGGCGGCCATACTGAGATGTTGTTGACACATGCAAGTAATTAAAGGAGGACACGTCGGAAGAATGAGATACTGGACAGG
TGGAAAAACTGTGAAAGCCTCTTCTGGTTTAGCGACGCTGTGAAAAGGCAAAGATTGGAGAATAAATGGAGAGAATTCGCATACAGGCCACAAGCTGTTC
CTCCCCAGGATTTAATAATTTTTCTCTGTATTTTCAACATATTTGGGATTACAATTTTTTTTTTTAAATTATTAAATCCAGATTTACAATATGTGTTTTT
TCCAGAACCATACGCTAAATTATTGGAACATTACAAAGACTGGGCTTTGTGGCTTGTTACAGCCTGTGTAATATAATGTCACTCCCTTTGTACAAAAATA
TAAATCCCCCAAAAAAATAATTTAAAAAAAAAATGACCCTGCTCTGAAATTTCAAATTTTGCAACCCTGTTAATAGGCACCTTACTGTTTTTTTTGCTTT
TTTGTTTGCATTTTTAGCTTCCATTTATGATCCATTTTTTTTTACATAAACTTTTGTATATTTTTTTTTTTTGTAAGATTTCCTGTGCTCGGTGTAGTCG
CTCAGTGACCTGCTGGGTCCCCACTGGAAAAAAACGTGTGCTTTATTTATATGTAAAAAAAATTCAGGGTGTCCGTATCCGCTGAAACTTGTACGCTCCA
AACGATACAAGACTATTGTTTACATGTTTATTTTATATCAGAATGAATTACTTTTATTTAAATGCATTTCCCCCTTAAACTGATTGGACAAGCTCAATCG
GATCTGCTCAAAGGAACATATTTATGTGTATAACATGGCCTTGATATGGTGCTCATTTTGCCCCCTTCATGCTCGAAATAAATTGCTGTAAAATTGCTCA
TATCACACTTCTGAGATCTTTTTGCAATCATTTTCTCTTGTTTTCAAGATATTGGCACTTTTTACACTGCTAATGCAATCACTTTGAAACAACAGCAGCT
GTCTACTGTTCATTTGCTTAACTTAAATCTGCGGAAAATTGATTTTATGAGAAGAGGGAAGTCTTGTGATGCTGCTCTGCTCAGAAAATGAGGTTAGTGA
GCAGAGAAAAGGCATCTGATTGTTATTTGGTTATTACTAAGCAGACTATCAATTCAATTTCCCAGATTCAATTCAGTGAGAAACAGTTATCTCCTGGTTT
ATCCCCGGAAAACTCATGGGTGAAATTCAATTCAAGATTCCTAATTGAGATCCAGTTTCTCCCTATAGACTTCAATAGAGTTTTTCACATGATAAACAAT
AAGATAAATGTTTCTGAATTGAATTGAATCTGGCCCAAAATTTGTTTTCAGCTGATGGGAGTTGACCAAAATGAACAGCAGGTGGTGCTGCTAATTCAGT
GTAAAACTGCTAATATCGTGAAAAATAGAAGGAATAATGAATGCAAACTGCAAAAGTGCTGAGAACAGCACTACATGAACTTCAGAATTACAAGGGTTAC
AAGCTGATCCTCCAAGATATTTGATTATGCTGAGATTTGTAGTTTCCAGCGGCTGAATAATTGGGTCAACTCATAGCAAACCAAAGCATGAGGTTGTTCA
GTAGATTAACCTACAGCCAATCAGAGAATTGGGGTGTTTACATTTAGACTAACCTGCATTTAATGAGTTTAGGGTGGGCATAAGTTCACACAATTTCCAA
GTCCTTGCCTCTCAGCAAGCTGGTTGGCTGCATAACTGGCCAATCAGGTTGCAGCCGCAGGTTCCTTTATAGCGCAGAATAGACTATTATTAAATCCCAT
TCATTCTTATGTTCCTAAACTGGAATCAGTAGAGCAGCATAGTGAGTGTTTCAGGGTGCTGAGCTTGGTGAAGTGATGGACTCAGCAGTCAGTATTAGAG
CGGGTGGGACAGGACTGGGGTCACGCATTTCGCAGTAAAACCCATGTCACTATATTAAGATCAAGTGGCAGTTAATCACACGGTCAAACCAACCCAATCC
AAGCAGTAGATTGTTCACATGATTAGAACAACTTCCAGCCAATCAGTTTATCAGAACCTTTCAGATATTGATACCAACCTGGGGCCAATCAGAGCGTTAG
GATGTTCAAAGTGCTCATCCCTCCTTTAGCCAATGAAAGCACTGGAACATACAGGACAGGTTGCTGACAGCACGTTTAGTTCAATGTATGAAATGGCCAA
AGTGTTGTTTTTTTAAAAAAAAAAAATATTTCAAAGAAAATATTTGCACTGAATTTACGCCTAAATCTTCCAAAAAAAAAAAAAAAA
5' EST: BI446109 [+]gcaagacttgtttgggcctcataaacaaccacagaaccacaagctgagcacagacactcgacagtggatccgggatattggtcagcagaaaggggcgaat
cattttcgttgactggaaataaaaaaaccacaagactttcatacattgctgattttttaaaaaaaaacactaaaaccccaggcaggaagttgaaggaaac
gcaatggcctcacatagtgccttccagtcatttcctttatacccgccgtgcttcttcagagataccagcaccagccgccgcttcacgccgccctccacca
ctctgagtccggggaagatgagcgaaccgataccgctgaatatcgcggacagcagcgccgccctggtgggcaaactgagaagcacagaccggaatatggt
ggaagtattgtccgatcatcccggagaactggtccgcacagacagccccaactttctctgctcggtgcttcccacacactggaggtgcaacaagaccttg
cccatcgctttcaaggtggtggccttgggagaagttccagatgggacactggtgacagtgatggctggcaatgatgag
3' EST: BI313873 [+]tggaagatttaggcgtaaattcagtgcaaatattttctttgaaatattttttttttttaaaaaaacaacactttggccatttcatacattgaactaaacg
tgctgtcagcaacctgtcctgtatgttccagtgctttcattggctaaaggagg
Library:
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.4 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
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Whole mount in situ hybridization:
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Expressed on Rohon beard cells, Embryonic haemangioblast, VBI, myeloid cells and DA-HSC.
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
SalI |
SmaI can also be used. |
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Suppliers:
Search for clone at:
EXRC
