Summary Attributions

Clone Name: dazap2-KL97	Gene: dazap2.S	Species: Xenopus laevis
Description: EXRC Clone Number KL-97. Xenopus Proline-rich protein, DAZ associated protein 2. Caused anterior defects and krox20 loss with ectopic staining. GeneBank BG354661. To generate mRNA for injection and probe for in situ. Clone previously called: 81C8A-81E10C

---

Sequence:

5' EST: BG354661 [+]

tacagagcgagcaacagacgcgaacccggagctatgaataataaaggacaatacccttctgccccagcctacccaacccaggcacccaattctcagtcag
tgtacccaccaaccatgcacctgccacaggctccttcttatacagatgcacctccagcttattctgagctgtaccgagctgcgtatatgcaacaagcagc
agctaatatgagcgctctatctgcacactaccccagcacatcaatgtaccttcctatggctcagcctatgcaagttgcacaaatgtcctcacaagtacct
atggcttattaccctattggtcctgtctacccacctggctccacagtccttgtcgatgggggctatgatgcaggggctcgatttggtgtgggtaactctc
catcggttccaccaccgccaaccggctgccccccgaatgcggctcagctggctgccatgcagggtgcaaatgtattagtcacacagaggaaaggcaatta
cttcatgggtggctcagatggtggatacaccatttggtgaaaaggaaacagaggcatggaaatgggggttaaaagcaaaaaaaaataaaaaaaaaataaa
taaaaaaaaaaagggcggccgaaggcctttcgagcctttcgccctataggagtcgtttacgtagatcccagacatggataaagattcctttggtggagtt
tgggacaaaccccaacttagaatgccggggaaaaaaaatgcttttatttggggaaaattggggatgcttttggcttttatttgggaaacccctttttaaa
gcctggcataaaacaaaggtttaaacaaccaacccaattgggcatttcatttttatgttttcaaggttccagggggggaaaggttttggaaagggttttt
t

---

Library:

Name: Harland stage 19-23 Xenopus laevis cDNA

External Dbs: Unigene laevis cDNA library

Description: cDNA made by oligo-dT priming. Library constructed by Dr. Francesca Mariani in the laboratory of R. Harland, Ph.D. (University of California, Berkeley).

Anatomy: whole organism	Stage: NF stage 19 to NF stage 23
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: NotI 3' Restriction Site: SalI

---

Usage:

RNA injection:
Probes:
Type Size Promoter Linearization Site Description sense SP6 AscI

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 SalI

---

Suppliers:

Search for clone at: Open Biosystems