|Clone Name: IMAGE:8916930||Gene: pa2g4||Species: Xenopus tropicalis|
|Name: NICHD_XGC_tropTe1||External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library|
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.
|Normalized: No||Subtraction: No|
|Vector Details||Vector Map||Name: pCS107|
5' Restriction Site: SalI
3' Restriction Site: NotI
Search for clone at: Open Biosystems Source BioScience