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Summary Attributions
XB-CLONE-3105910

Clone Name: dll4-891 Gene: dll4 Species: Xenopus tropicalis  
Description: EXRC Clone Number 891. Xenopus tropicalis delta-like 4. Full length. To generate probe for in situ. Clone previously called: IMAGE:7876232

Sequence:

5' EST: DT435811 [+]

3' EST: DT435810 [+]

Unigene: Str.59435 , Str.87478


Library:

Name: NIH_XGC_tropSki1External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1562 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Anatomy: skinStage:
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image

Usage:

Whole mount in situ hybridization: Arterial Specific  
 
Probes:
Type Size Promoter Linearization Site Description
antisense T7 EcoRI ClaI or BamHI can also be used


Suppliers:

Search for clone at: EXRC

Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.12.0


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