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XB-CLONE-3134988
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Clone Name: CAAO3987
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Gene:
ccnb1.2
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Species: Xenopus tropicalis
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Sequence:5' EST: CX934747  [+]cggagaggtttgtagtgaatcagttgccgttaccagtttggaaaaatgtcgctgcgtatcaccagaaacatgatggcaaatgcagaaaacaatgtgaaaa
ccactgtagctggaaagagggttgttgcaaccaaaccagttttgaggcctcgtacagctttgggagacataggaaacaaggccgagctaaaagttccagc
aaaaaaggaactgaagccagcagtaaaagctatcaagaaggtaaaacctgtggacaaagtgttggagcccctgaaagttagagaagagaatgtttgcccc
aaacctgctcaggttgaacctagctcaccaagcccaatggaaacatctggttgcctccctgatgagctctgccaggccttctctgatgtcttgattcaag
ttaaagatgttgatgctgatgatgatggcaatccaatgctgtgcagtgaatatgtgaaggacatttactgttacctgaggagcctggagaatgcacaagc
agtcagacaaaactacctacatggacaggaagtcacaggcaacatgcgtgccatattgattgactggctggtccaggtgcaaatgaaatttcgtctgctg
caggagactatgttcatgactgttggcataattgatcgctttctgcaggaccatccagttcctaaaaaccaactgcagcttgtgggggtcacagctatgt
tccttgctgctaaatatgaagagatgtacccaccagaaatcggagactttacatttgtaactgatcacacatacacaaaggctcaaattagggacatgga
aatgaaggtgcttcgggtgcttaagtttgca
3' EST: CX934746 [+]ctcccatgagggggcgggccaattccacaacaacatctgatctcagctgggggaacatgctgatcttcatttgtttgctgctggcatatttgttcttaac
agtcaggggtttggtcagtcccttgttcaccttaaagatgttcttggccatatgttgcataacagggacaaaatcatcttcaaagtaagccataaaatga
tgcattgttggggtccattcccccgtattcaaaattttaaaaaacaagcaaaaggcagcacctgctatttgggaaggcgagtaatgccccatatcataat
cccccattacaagttccattaaatatttagccaaactgggctgttcagcagtcacctctccaatcttaaaagctctcccaaaaaagtgcaaaggtaatgg
tcggcctattgcaaacttaagcacccgaagcaccttcatttccatgtccctaatttgagcctttgtgtatgtg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.
| Anatomy: testis | Stage: frog | | Normalized: No | Subtraction: No |
| Vector Details | Vector Map | Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
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