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Summary Attributions
XB-CLONE-3152581

Clone Name: pax8-HAR131 Gene: pax8 Species: Xenopus tropicalis  
Description: EXRC Clone Number HAR-131. IMAGE:7863442. Xenopus tropicalis paired box 8. Full length. Accession number DT412463. To generate probe for in situ. Approximately 950bp of x.trop pax8 was amplified using the following primers: ggaattccGGTCACGGAGGTTTGAATCAGTTAG cgggatcccgTGGGGCTACATAAAGATGAAGGG which also places an EcoRI site at the 5'end and a BamHI site at the 3'end. This was amplfiied using Pfu from 25-30 stage x.trop cDNA Amplicon was cloned into the RI-BamHI site of pBS KS II.

Sequence:

Full length sequence: BC125805 [+]

5' EST: DT412463 [+]

3' EST: DT412462 [+]

Library:

Name: NIH_XGC_tropOvi1External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Anatomy: oviductStage:
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI		Expression Image

Usage:

Whole mount in situ hybridization:  
 
Probes:
Type Size Promoter Linearization Site Description
antisense T7 EcoRI

Suppliers:

Search for clone at: EXRC