Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Summary Attributions
XB-CLONE-3179390

Clone Name: IMAGE:7853572 Gene: map3k7 Species: Xenopus tropicalis  
 

Sequence:

5' EST: DT394869 [+]

3' EST: DT394868 [+]

Unigene: Str.18072


Library:

Name: NIH_XGC_tropSkeMus1External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).

Anatomy: skeletal muscleStage: frog
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image

Usage:


Suppliers:

Search for clone at: Open Biosystems Source BioScience

My Xenbase: [ Log-in / Register ]
version: [4.6.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556