XB-CLONE-3191944
Clone Name: IMAGE:7887495
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT386597 [+]
tgggtgcaggacggcgacgccgcgctgacggcgctcggcggcgagacgtacgacctgctgctgctcgacctcggcctgccgaagcgcgacggcatcgacg
tgctgcgcacgctgcgcgcacgcgggctggcgctgccggtgctgatcgtcaccgcgtgcgacgcggtggccgatcgcgtgaaggggctcgacgcgggcgc
cgacgactacctcgtcaagccgttcgacctcgatgaactgggcgcgcggatgcgcgcgctgatccgccgccaggccgggcgcagcgaatcgctgatccgc
cacggcgcgctgacgctcgatcccgcttcgcaccaggtgacgctcgacggcgtgcccgtcgcgctgtccgcgcgcgagttcgcgctgctcgaggcgctgc
tcgcgcggcccggcgcggtgctgtcgaagagccagctcgaggagaagatgtacggctggggcgaggagatcggcagcaacaccgtcgaggtttacatcca
cgcgctgcgcaagaagctcggctcggacctgatccgcaacgtgcgcgggctcggctacatggtcgtcaaggaaagctgacgcgtgaggtcgattcgtcat
caattgctgatctggctgctcgcgatcgtcgttgcgggcgtgggcatcgcgggctggctcatctaccggcaggcgctcgccgaggcgaacgaactgttcg
actaccagttgcaggaaatcgccgcggcgttgccgtccgag
3' EST: DT386596 [+]agccgcgcgagcagcccgttcagtgcccggacgagcggctgcacctcgagcggcaggcgcccatcgggcagcggatcgagcgcttccggccggcgcgcct
cgaccgcgcgcgtcacgcggccgagcggcgcgagcccgcgcccgacgatcccccacacggccgcgccgaggaacggcaacagcacgatcagcggccacag
cgtgcgcagcgcgacgttcgccgcgagccggttgcgcaccgacagcggctgcgcgagctgcacgacgttgtcgccgacgatcgcgccgtacacgcgccat
tcgccgcgatcggtgcgttcggtcgagaagccgagctccgcgcgcggcgcgatcggtgcacgcggatgcgagaagtacatcagcaccccgttgcggttcc
agatctggatcacgatgccttcttcgccgttggtgcgcgaaccgaacacctgcgagaacggctcggacggcaacgccgcggcgatttcctgcaactggta
gtcgaacagttcgttcgcctcggcgagcgcctgccggtagatgagccagcccgcgatgcccacgcccgcaacgacgatcgcgagcagccagatcagcaat
tgatgacgaatcgacctcacgcgtcagctttccttgacgaccatgtagccgagcccgcgcacgttgcggatcaggtccga
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis (1.5-2.5 kb fraction; NIH_XGC_tropMet10 contains the 0.5-1.5 kb fraction) and ligated into SalI and NotI digested pCMV-SPORT6 vector. Primary library, not amplified. Library constructed at the DOE Joint Genome Institute.Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: