|
XB-CLONE-3259948
|
Clone Name: IMAGE:7684257
|
Gene:
ranbp2
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:5' EST: CX902654  [+]cgataaaggccgcgtaacttgcccagcatgaggcgaagtaaagcagagctccagagatacatagaaaatgcgcagaacagcgcgacatcacccagagaga
aatcaatgaaaggatttctttttgctagactctactatgaggctaaagaatatgaacttgctaaaagatgtgtgtcttcgtacattagtgtacaggaaag
agacccaaaagcccacagattccttggacaactgtttgagattgaaggaaatgtggaaaaagctgttggctgttacaagcgttcattggagttaaacccc
actcagaaggatcttgttcttaggattgctgagttgatatgtactctaaatatcaaagatggcagagcagaatactgggtggacagagcatccaaactct
tcccaggaagtcctgagatatacagacttaaagaacaactactttcctcacaaggagaagctgggtggaatcagctcttcgatttaatacaggccgaact
ttttgcaaggcccaatgatgtgtatgttaatatcaagcttgtagatttgtttctgtccaaccaaagactggaggaagcagtttggcattgccttaaaccc
gagagaagagctttaaggacagatttagaatggtgctcccgtgttgtccaagtatttaaggagtatctggcttcaaaacaagggcaaaagaacaccaatg
tgaagacaataacaaaggaactgttgcttgcgcattctgatngtgtccttttgactctttctaaaaaggatgtttggaagagtaaggaagccc
3' EST: CX902653 [+]ttttttttttttttgtagtagtcttagctgtttcagactctttatcgtttgtggaagtacccttcatgaagccagcagacatttcctttgtcttatttga
atcagatattccaaaattaaaagtaagaggaccggcagacaatggaaagttaaagccctcttttaccacctttgattcatcattggaaacaggggcttga
aacgcaaagtttgatgctgatgatttttcgtcactaataccaaatttgaacccttttccagagaaatcacaattaaatcccgcaacaggttgcccaaaat
tctgtgatgaatttgctttaataccaaaagtaaaaggcacctccttgctttgactggcagatgagccaggtttagcagtctggcaagcagcacaattttc
ttttgaagcatcgtttataactaagcatgtactgcactcccactgaccctctttgcgtgaaaacatagctgataaactcggttgtgttttaccaaactct
gcataaggcccaaatttgaagccagatggggtctgggcaggtggtacagcatctttatttgttgcctgcggattggcactctggcaagctacacagtttg
aagcagatgcttcatttctgacaagacatgaattgcaatcccattgtccttccttctttgcaaactgggcaccaaaatttttttgtgaattatcagcacc
aggagcaaaagaaaaagatgctgcttgggcaacagctttactcaatggctttgtattttgacaagaaacacatttatttgctgaaggttcatttctaaca
taacatgcatcacaatcccactgacctggtttttt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.
| Anatomy: testis | Stage: frog | | Normalized: No | Subtraction: No |
| Vector Details | Vector Map | Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
|
