Clone Name: IMAGE:7674525
Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-449.
Xenopus tropicalis H6 family homeobox 3.
To generate probe for in situ.
Sequence:Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.
|Anatomy: brain||Stage: frog|
|Normalized: No||Subtraction: No|
|Vector Details||Vector Map||Name: pCMV-SPORT6|
5' Restriction Site: SalI
3' Restriction Site: NotI
Whole mount in situ hybridization:
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