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Summary Attributions
XB-CLONE-3461271

Clone Name: IMAGE:7749932 Gene: pa2g4 Species: Xenopus tropicalis  
 

Sequence:

5' EST: DN048717 [+]

3' EST: DN048716 [+]

Unigene: Str.7703


Library:

Name: NIH_XGC_tropKid1External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library.

Anatomy: mesonephric kidneyStage: frog
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image

Usage:


Suppliers:

Search for clone at: Open Biosystems Source BioScience

Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.12.0


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