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Summary Attributions
XB-CLONE-3677463

Clone Name: IMAGE:7517841 Gene: nkx2-6 Species: Xenopus tropicalis  
Description: EXRC Clone Number 911. Also Known as NKX2.10 for WISH.

Sequence:

5' EST: CX749505 [+]

3' EST: CX749504 [+]

Unigene: Str.54133


Library:

Name: NIH_XGC_tropNeu5External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGCTCT(20)V-3'). After ligation of PstI adapters and XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Anatomy: whole organismStage: NF stage 14 to NF stage 18
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS22+

Type: plasmid

5' Restriction Site: PstI

3' Restriction Site: XhoI
Expression Image

Usage:

Whole mount in situ hybridization: Expressed in Heart.  
 
Probes:
Type Size Promoter Linearization Site Description
antisense SP6


Suppliers:

Search for clone at: EXRC

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version: [4.6.0]

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