XB-CLONE-3916565
Clone Name: IMAGE:7091362
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Gene:
pak2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653739 [+]
taattattcccccccccccccgctctgaaccttctacagcagcaacagatgatgatagcgatgatgataaggctcctcctcctgttattgctccaaggcc
agaacacaccaaatcaatgtatacacggtctgtaattgacccaatacgtccaccccctggagattcagacagtgctgcaaaggctggagaccggcagaaa
aagaaaacaaagatgagcgatgaagagattatggaaaaacttagaactatagtaagcataggagaccccaagaaaaaatatactagatatgaaaaaattg
gacaaggggcctctggaactgtatttactgctattgatgtagctaccggacaggaggttgcaatcaaacatataaatcttcagaagcagcccatgaaaga
actgataatcaatgagattctagtgatgaaagaattgaagaaccccaatatagtatatttcctggacagtttcttggtgagtgacaagctgtatgttgta
atggagtatttggctggaggatcccttacagacgtagtctcgtaaacctgtatggatgaggctcaaaagctactgtctgcacaaagtgtctgctaacttt
tgaattctaacttgccaaccttgtcttttcataaacgtttagagtgcaagttctccttggagggatggtccgtcaactg
3' EST: CN320812 [+]tttttttttttttttctagtttatgcaggtactggctatctgtaaaaattggttttaagcacataaagaaatatcaggacacaatctgagacaacactgc
ttgtaaaaaaaaaaacaaaaaaaaaaaaaaaatatgcacttcaagttcatcaaacaacccccaatgctttcagtacacagtaaaaaaaataaaacatgac
tatgaaatgactccgacgtacagtaaaactggttctgtcacactaaaaagcaataaaaaatggcactgtttagcagcaggaaaaaaagtgaaatgaatgc
aattctgaccccaaaacggaactgctgctggtggatcggcctctctattggcacaaaaaatgggacgctggaactctaatttcccctgtgccactgccta
ggtaaaagttgcataattttattctgcttaaaacccacataactcgtatttaagtaaaaaataaaaaatacacaaagtgggtgaacgatctctatgggcg
gttcaacaccttagcaccaggaaataaaaagctatcgtctcttttgttctgcgagcgcatagctcctgcacaccaactgctggcagccacatttaacttt
cctaagccacatatttcctaggctatcaccttagatctgtctctaaaaaaacatgaatgtgagcattatagacttacatctaatatagattaaaccattt
gtattaacacaggtttaatcttaaattctctttactaacaggagacaaacataattttcaagaaatggaaaacacataatcatattaatacaagggaatc
acggattacctttcctttccaaaaatgggtaaaaaaaacgtggaacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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