XB-CLONE-3944877
Sequence:5' EST: CN103336 |
|||||||||||||||||||||||||||
| Name: Xenopus tropicalis xthr plasmid library | External Dbs: Unigene tropicalis cDNA library |
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).
| Anatomy: head | Stage: NF stage 25 to NF stage 35 and 36 |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map | Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: SfiI 3' Restriction Site: SfiI |  |
Usage:
Suppliers:
Search for clone at: open biosystems geneservice/RZPD imagenes