Summary Attributions

Clone Name: twsg1-HAR91	Gene: twsg1	Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-91. Xenopus tropicalis twisted gastrulation homolog 1. Accession number AL634092. TGas012m12. To generate probe for in situ.

---

Sequence:

5' EST: AL634092 [+]

tttggtggccaagtctgaagccctctctcctacccattccagcagcagctctgctcctctgctctttctggattttgcctgttcgttgctgcaataaggc
tctgtgtgcaagcgatgtcagtaaatgtttattacaggagctgtgccattgtcggcccatggatggaaactgctcctgctgtaaggagtgtatgctgtgc
ctgggcacactctgggacgaatgctgcgactgcgttaatatgtgcaacccaagaaactacagcgatacacccccaacctctaaaagtacagtggaggaga
tttatcaatccattccatccctgttccgggctcttactgaggaagacacccaacttaactggaatattgtaaccttccctgtagtggaagagctttccca
tcatgagaacctggtctccttcctggaaactgtaaaccaaccacaagagcaaaacgtatcagtcgaggtctctcacagcaatgacaaagagcacatgtgc
acagtagtgtactttaatgagtgtatgtcaatccatcagtgcaaggtttcctgtgagtccatgggagcctcgaagtaccggtggttccacaatgcttgct
gtgaatgtgt

---

Library:

Name: XGC-gastrula

External Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organism	Stage: NF stage 10.5 to NF stage 12
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

---

Usage:

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 ClaI

---

Suppliers:

Search for clone at: EXRC