XB-CLONE-4350132
Clone Name: IMAGE:7025777
|
Gene:
hoxb9
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785719 [+]
taattaatcccccccccccccgccgattgatgaatagccctctgacgctttatcaggcacacggagaaagtttgaccaatcatttgccaggagctcacac
gccagcagcccaagtgcaccgagttggcgagcaagttgggagaggggagagctcctatatcttgttcccttcaatgaagctgctgagtaggatgcccgag
aatggccatttctgggactctcagtaattattttgtggagtcaataataagccctgagagtgaggaggctccagcagccaaattctctggacagtacccc
agcccccggccagcagcccattcagctcactctgagcccttggactttccctcctgtagtttccagcccaaagctcctgtattcagcgcgtcctggagcc
ctctgaacccccacccagccagccccctgccttctgtctaccacccctacatccagcagggcgcccccactccggagggcaggtacttacggggctggct
ggagccgctgcaaagagcagagaatggacctggccaaggaactgtgaagtctgagccgctgctggggcccccgggggagctggacaaactgggagcccaa
cagtacaatctgggctcgcctgctgcaagagaagatgccaatgaaagggccacttttccggacaataaactgtgtgaagggaagcggggacaaagacagg
acacatccaaagcaaccccctcagccaacttgggttacatggcccgccctccttccaggaaaggaaaaggtggccccctacacccaaaataccaagaacc
cctgggaaactggggagaaaagggaattt
3' EST: CF785718 [+]tttttttttttttttggagtcactaatttaataaccatggaaataaaaacatcatgaactccccccccgcaactgtaaacagtgtaaaatgacaaaatac
cttgaaaaaaaatacaaactacatttatacacacagagatttcaagtataaagtcttcaacaggaacatacaaaatatacagtcacttgtcacaaggaac
aatttctacatcttgtaaaaaggtaaattcacaataaattataaatatctacttctagggatacattcactttttcccaccctcttctaaaacaaaacaa
acaaaaaaaatcctttttttttacattttttttttaacccttggtttctacaaaactaattcagcaaagcgagggcgtttcttgtgcttctatttaacct
cttctctgtctctctcaaacgttttatattcaagctcagtataaaaatgggagtcttgctggtttcaaggccctttaataagagatgcggttgggatgaa
atatatatatgcctctttatggggattattgatttatggtatgtggtggcgatatgtcttggttggctgcagtctttatgtgggtaaatagccttgttta
aaagcagccgtagccttgtgcttgaaaggatcatgcaaggtaagtgccacctttgtgcagtcagtttctttcactggaccttcacattgtaccataaata
aggaccaggggaagggaaggggtaaaccttggctccgatctttgttaagtttcctcatcttcattcttcgggtccgggaacccaatttggac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: