XB-CLONE-4350443
Clone Name: IMAGE:7025655
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Gene:
nckap1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785523 [+]
taattaatcccccccccccccttcttggcgcttgcatcctccaggctgctaaagattggccaagagactgacaaaaatactacaagaaacagggaatctg
tttatttgctgctagatatgattgttcaagaatcaccattcctgaccatggatctgctggagtcctgcttcccatacgtcttactgagaaatgcatacca
tgcggtttacaaacagagtgtcacatcctctgcataaatatttactttgtggaataaaccggcgcacaattagaatgcctcagttttctcccagaaactg
tggtggagtacttttactgaatagtttggaaaaacaaaacaaacaaacaaaaaaaagaaaaactgttttgtgactaaaactctatccctattccattgtc
tttttgaccagtggtttcttgatgcagttgtcctgccagataagcttggttggttgcacttcactaaggaaaaaaaaaaaaaaaaactatttgtttttga
acggttacattaaggaaaaaaaaatcatgaaaaatggtttcagctgcgctacttttgctctgttttgatatctgatcattctaaatattattgcttgttt
atttcaaagaaaaaaatgtatttagtgattattttagatgggtgatataggtgaacatcttcgtgtaaattatttcatacagggagaaattctaatcnaa
taccctttccttttaaggtccttaccggatgacaattttggggtaaggtgaattcctgtgaattttccgttgaaagtaattgaaacccatggtaacctaa
cccccttccaattccaattttccaactcgccccccaaaa
3' EST: CF785522 [+]tttttttttttttttgcaagcgttttgggcgagtggaatggagtggaggggtagttacatggttcaattacttcacgggaaatcacagaatcacctaccc
aaattgtcatcagtaagaacataaaagaaaggtatagattagaatttctcctgtatgaaataatttacacgaagatgtcacctatatcaccatctaaaat
aatcactaaatacatttttttctttgaaataaacaagcaataatatttagaatgatcagatatcaaaacagagcaaaagtagcgcagctgaaaccatttt
tcatgatttttttttccttaatgtaaccgttcaaaaacaaatagttttttttttttttttcccttagtgaagtgcaaccaaccaagcttatctggcagga
caactgcatcaagaaaccactggtcaaaaagacaatggaatagggatagagttttagtcacaaaacagtttttcttttttttgtttgtttgttttgtttt
tccaaactattcagtaaaagtactccaccacagtttctggggagaaaactgaagcattctaatttgtgcgccgggtttattccacaaaagttaaatattt
tatgccaaaaggaagtgaaccactcctggtttgggaaaaccccccagggtaatggcattttcctccattaaagacgttatgggggaaaagcaggggactc
ccaggcaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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