XB-CLONE-4350458
Clone Name: IMAGE:7025660
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Gene:
aldob
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785512 [+]
taattaatcccccccccccccgctcgtcacacctaggagtttctactggcgacttctctcacagcaaattaagcacaatggccccggtattcccagcact
aactgcagagcagaagaaggagctgtctgagattgcccagcgtattgtggcaccaggcaaaggaatccttgcagcagatgagtctgtggggactatggga
agccgcctcaagcgcatcaatgtggagaacatagaagagaacagacgtctctatcgagagcttcttttcactgcagatccctctataaaagacagcattg
gaggtgtcattcttttccatgaaactctctaccagaagtctcgagatggagttctttttcccaaaatcctcaaggatcttggcattgttgtaggaatcaa
ggtagataaaggcactgctcctttagctggaactgatggagagaccacaacacagggtttggatgggcttgcagaaagatgtgctcagtataagaaggat
ggcgcagactttgcaaagtggcgttgtgtgctgaagatctcggattctgcaccatcaagtcttgctatctatgagaatgctaatgttttggctcgatatg
ccagtatctgccagcagaatggacttgtgccaattgtgggaacagaaatcctgtctgatgggtcacatgatctccaaaggtgttcagtacgagactgaaa
aggcccggtccgctgtggtagccgccctaattccgcaccatgttttatcctggaaggaactctatttaaaaccaaattgg
3' EST: CF785513 [+]tttttttttttttttgtatgtaaagatcaattttaatgtggcagaaatacattttggaataatacaagaagtaaaaaggtcaaactgatgtgattttgtt
gcacactaaatattatttatgaatttgtatacagtatttatctcttgctcaaatgttggaactactttgagtacaaagagggctgaaataaaattgaaat
atacagattttggcccataccagtaaaaacctatggtacttaaacaattggcatacaaataatctcattctttagaaaatactatttggaatgttggtaa
atctgtcattagggcatggattggagactggtcaccaaatgcagattttggtattttgtgcttttaacaactggaatagatcacagacaggctgtttaat
aggtgtaactaggctgaaacagagactgagatgaagcagaatctgaagatccagatggaacatattttccaagagttgccaaaccattaatctttgccct
cttaacaaaggcttcctgggcagctttctcattctcactcttcccaacccaggcagagagtgctgatgcctgtagagcacgaccgtatgagaaagacaca
cgccatggccgattaagactgcagcaattcatagcattcaggttaagggaggcctcttcttcactttgacctcctgagaggaagcagatacctggaactg
caggaaggactgtacgtcttaaagcagtgacagtggccatggcaacatcttgtggggtatatttttgtgaacaagactgggcccctgtgaacataatttg
gttttaatagaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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