XB-CLONE-4350469
Clone Name: IMAGE:7025664
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Gene:
srsf6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082527 [+]
ACTCTGCTGTGTTTGGAGCTTGAGTGTGAGACAACAGTCGCCATGCCGCGGGTGTATATCGGAAGGCTCGGCTACCACGTACGGGAGAAGGATATCCAGC
GGTTCTTCGGGGGCTATGGGAAGCTACTAGAGGTTGACTTAAAGAACGGGTATGGCTTTGTGGAGTTTGAGGACTCGAGGGATGCTGATGACGCAGTGTA
CGAGCTAAATGGCAAAGATCTATGTGGCGAGCGCGTGATTGTAGAGCATGCCCGGGGTCCACGGCGAGACCGGGATGGATACGGCTATGGAAGTCGCAGT
AAGCATTGTAGTCCTGGGTAGCAGCAACTTGAGACCTCTTTGCATTTCACTTCAAATACTGGGATTCTTTCTGTTGAAACTGAGGCCAGAAGTAGGGGTT
TAATTTCTGTGAGCCCTCCGCCTCTTTGTGGGAAACACATTGTGACCAACTTTACCTTCCAGACAGGACCGCCATCAGTAATCATGGGGCCCCGTGCTAC
TTAGTTACCCCCCCCCCCCCCCCCAAGGAACTGCAATTAGCTCATATGTCACCTGAGCAGTGGGCTGTGGCAACAATTAGAATAGGGTCCCTACTGGTCC
TAATTTGTGCATGAAAAGGGTGTTCCTAATTTGCCCAAAACAACCCACAAATTGCTCTTTTGGCCCCCTTCTGCCATTGGGATATTGATAGGAGGAGTGC
CCTGTGTCCCCCAGATGGCGGCCCTGCTTCCAGGGTTTAGGTCCTGTGTTTAACCTGTTTTTTGTTGCCTGACAACACGCAGAGTTACATGGGTCGGCTC
TCAACTCAATAACATAAGTTAAAGCATCGGCAGACAATTAAAGCTGACTTTGACCCAACTTTATATTAAGAATATTTAGCTCTTAATATACAATTTAAAT
CCATAGGGATGTCTCCTTTTATAAAGTTAGGCGTATTGGACTGGAAACATAACAGCAGTTCTTCAGTTTGTACCCTTTTTTGGGCCTTTGCCCTGTAACA
CTTCTTTCATTAATTTATAGCACAACTTTACATTACTGGTACTGCACTTATAGCAATGTGTAAAAAAAAAAAATGTTGGCAGAAGAGATTAATTGGGAAT
ATAAGGTTGGATGTTACCTGGAAATAAATATATACAGCTTTATTTTTCATTGGCTTTTATCTTACCTCTCAATCCAATATTGGAAAGTTAACCAGGAATG
TATGTGTTCTTGGTAGACTGGTCAAATCAAATGCATATGGGTTCAGTATTGGTTGAGACATTAGTTTTTCTAAGGGGTTGTTTGTCTTTGAGTTAACTTT
TGGTATCCTGTGGAGTGTAATATCCTGAGACAGTAGCAGCTGTAAAAAATGTTTGTTTCAGTTTGGATTTTCAGCAGCTATCTGGTTGCTATGGTCTAAG
CTACCTTAACAACCAGAGTTGTTTGAATGAGACTGGAATATGAATAGGGAGGGCCTAAATAGAAAGATAAGTTATACAAAGTAGCATTACGTTTAGGGCA
ATGTTTTTTGGCTGCTGGGGTCGGTGGCCCACATTAGAAAACTGAAAAAAAAAAAAAAAA
5' EST: CF785505 [+]taattaatcccccccccccccactctgctgtgtttggagcttgagtgtgagacaacagtcgccatgccgcgggtgtatatcggaaggctcggctaccacg
tacgggagaaggatatccagcggttcttcgggggctatgggaagctactagaggttgacttaaagaacgggtatggctttgtggagtttgaggactcgag
ggatgctgatgacgcagtgtacgagctaaatggcaaagatctatgtggcgagcgcgtgattgtagagcatgcccggggtccacggcgagaccgggatgga
tacggctatggaagtcgcagtaagcattgtagtcctgggtagcagcaacttgagacctctttgcatttcacttcaaatactgggattctttctgttgaaa
ctgaggccagaagtaggggtttaatttctgtgagccctccgcctctttgtgggaaacacattgtgaccaactttaccttccagacaggaccgccatcagt
aatcatggggccccgtgctacttaattacccccccccccccccccaaggaactgcaattagctcatatgtcacctggaacaatgggcctgtggcaaccat
taaaaatagggggcccctactgggtccataatttgtgcatggaaaaggggtgttccctaattttgcccccaaaaccaaccccacaaaattggctcctttt
gggcgcccctttctggccatttgggggatatttgtataggcagggaatggccccctggtggtccccccaaaagggggggccccttgcttcccaggggttt
aaggggccctgtggtttacaccc
3' EST: CF785506 [+]tttttttttttttttcagttttctaatgtgggccaccgaccccagcagccaaaaaacattgccctaaacgtaatgctactttgtataacttatctttcta
tttaggccctccctattcatattccagtctcattcaaacaactctggttgttaaggtagcttagaccatagcaaccagatagctgctgaaaatccaaact
gaaacaaacattttttacagctgctactgtctcaggatattacactccacaggataccaaaagttaactcaaagacaaacaaccccttagaaaaactaat
gtctcaaccaatactgaacccatatgcatttgatttgaccagtctaccaagaacacatacattcctggttaactttccaatattggattgagaggtaaga
taaaagccaatgaaaaataaagctgtatatatttatttccaggtaacatccaaccttatattcccaattaatctcttctgccaacattttttttttttac
acattgctataagtgcagtaccagtaatgtaaagttgtgctataaattaatgaaagaagtgttacagggcaaaggcccaaaaaagggtacaaactgaaga
actgctgttatgtttccagtccaatacgcctaactttataaaaggagacatccctatggatttaaattgtatattaagagctaaatattcttaatataaa
gttgggtcaaagtcagctttaattgtctgccgatgctttaacttatgttattgaagttgaaaaccggacccatgtaaacttcttgcgtgttgtccaggca
acaaaaaaaaaagggttaaaaacacaggggaccctaaact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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