XB-CLONE-4350485
Clone Name: IMAGE:7025673
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Gene:
capn1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081353 [+]
AGCGAGAGACTCCCGGCACAGAAGGCTCCCCTCCACAGCTTTGACCCTCCCTGGGAATTGAACCCAGCTCGCCTGCTGTTGTTGCTGAGATGGCTGAGGA
AGTGATTGTGCCCGTTTATGCCTCCGGGATTTCGGCGCAGCTGCAGAAGCAACGTGACCAAGAGCTGGGTGTTGGGAAACACGAAAATGCAGTTAAATTT
CTGGGACAAGATTATGACCGGATTCTCCACGAGTGCCTGCAAAACAGGACCCTCTTTAAGGACGACACTTTCCCACCCTCTGCTTACTCTCTGGGCTTTA
AGGAGCTCGGCCCAAACTCATCCAAAACCTATGGAGTAAAATGGGTTCGGCCTTCGCAACTCGTCTCCAACCCACAGTTTATTGTCGATGGTGCCACAAG
AACAGACATCTGTCAGGGAGCCTTGGGTGACTGTTGGCTCCTCGCTGCTATCGCCTCCCTCACATTAAATGATACAATCCTGCACCGTGTAGTACCTCAT
GGCCAGAGCTTTCAGGATAATTACGCTGGTGTCTTTCATTTTCAGCTGTGGCAGTTTGGCGAGTGGGTCGATGTGGTTATCGATGATTACCTCCCTGTTA
AAGATGGAAAACTTGTGTTTGTGCATTCGGCTTCCGGGAATGAATTCTGGAGTGCCTTGCTGGAGAAGGCCTATGCCAAGGCCAATGGTTGCTATGAGGC
TCTTTCTGGAGGCAGCACATCAGAAGGTTTTGAGGATTTTACAGGAGGTGTTACTGAGTGGTATGAGATGAAGAAAGCACCAAAAGATTTATTTAGCATT
ATCATGAAGGCTGTGGAGAGAGGTTCCCTCATGGGCTGTTCCATTGATATCACCAGTGCCACTGATATGGAAGCTGTAACATTCAAGAAGCTTGTAAAAG
GCCATGCATACTCTGTTACTGGAGCCAAAGAGATCAACTTCAGGGGTCGGAGCCAAAAGCTGATTCGAATGAGAAATCCATGGGGTGAGGTGGAGTGGAC
AGGTGCTTGGAGTGATAATTCATCTGAATGGAATTCTGTGGATGCAGCAGACAGTCAAGACTTGAGAATAAAAATGGAGGATGGAGAATTTTGGATGGCT
TTTGAGGACTTCCTTCGTGAATTTTCTCGCCTTGAGATCTGTAACCTCACACCCGATGCACTCACTGCCCGCAAATACCGCAAGTGGAACACAACCGTCT
TTAATGGGAACTGGAGGAAAGGTAGCACAGCTGGCGGATGCAGAAATTTCCCTGCCACATTCTGGATTAACCCACAGTTCAAGATAAAATTGGATGAGGA
GGATGATGATGATGACTTCGGCTCAGAAAAAGGTTGCACATTCCTGCTTGCACTTATGCAAAAGGACAAACGCAAAGAGAGGCGCTTTGGGAAAGATATG
GAGACCATTGGATTTGCAGTCTATGAGGTTCCTTCTCAGTTTCATGGACAGTCTGGTGTGCACCTAAAGCGGGACTTCTTCTTACAAAACGCCTCCCGTG
CTCGCTCCGAGCAGTTCATAAACCTGCGAGAAGTCAGCACCCGCCACAAACTGCCCCCCGGAGAATACATTGTGGTCCCTTCTACCTTCGAACCTAATGT
GGAAGGAGACTTTGTTGTTCGATTCTTTTCTGAAAACAAGCATGGCTCTGTTGAGATGGATGATCCATTATCTGCTGACCTACCTCCTGAGGAAGTACTG
AGTGAGGCTGAAATCGATCAAGGGTTCAAGAACCTTTTCAAGCAGCTGGCTGGTGCTGATAATGAAATCAGTGCTACAGAGTTACAGTCCATTTTAAATA
AAATTATGTCAAAACATAAAGACCTTCGCACATCGGGATTTAGTTTGGAGTCGTGCCGCAGCATGGTGAACCTGATGGATAAAGATGGGAATGGGAAATT
GGGACTGGTGGAGTTCAATGTTCTGTGGAATAAAATTAAAAATTACTTGACAGTATTCCGAAAGTTTGACATGGATAAATCTGGAAGTATGAGTGCCTAT
GAGATGCGTTTAGCTGTGGAGTCTGCAGGTTTCAAGTTAACCAACAGTCTGCATCAGCTCATCATCACCAGATACTCAGAGCCTGATATGGCTGTGAATT
TTGATAGCTTCACATGCTGTCTCATCAGACTGGAGACTATGTTCCGTTTTTTCCAGGGTATGGACACAGAGAAGAAAGGAGTGATTCATTTTGACATTTT
TAAATGGCTCCAAATGACAATGTTCGCATAGAAAAATCCCAATTCTGCATGGGTTGGGTCCCCCAACGCTCCTGTGACTTTCTGCGAGTGCCTTTCAACT
GCTGCTGCTGCTGCTACTGCTGCTGTGGTGCCAAAGCAATACTATAAGGATTTCTCTATCTGCACTCTCAGGCATCTGCTCCCACGTGGTATCTGTTTTA
TCACTCTCACATCCCCTATCATTCCCCTTTCTCTACCCTACCAACCAGTCAGACTGCTCTTCTCTCACCTCTGTAGTTAGCGTGCAAAGATAATCTATCC
TCTTGTCACCAAAATGTATTTTGGGGATACAAATATGCCTCTTTAGGTCATGCCTGTGACCCAGATATCTGTAGTTACTCTTATAGTGTCATTTTGTACT
GGATTTTAGATGCCAAAAATAAAGGCTCATTCTGTAATGTGAAAAAAAAAAAAAAAA
5' EST: CF785496 [+]taattaatcccccccccccccagcgagagactcccggcacagaaggctcccctccacagctttgaccctccctgggaattgaacccagctcgcctgctgt
tgttgctgagatggctgaggaagtgattgtgcccgtttatgcctccgggatttcggcgcagctgcagaagcaacgtgaccaagagctgggtgttgggaaa
cacgaaaatgcagttaaatttctgggacaagattatgaccggattctccacgagtgcctgcaaaacaggaccctctttaaggacgacactttcccaccct
ctgcttactctctgggctttaaggagctcggcccaaactcatccaaaacctatggagtaaaatgggttcggccttcgcaactcgtctccaacccacagtt
tattgtcgatggtgccacaagaacagacatctgtcagggagccttgggtgactgttggctcctcgctgctatcgcctccctcacattaaatgatacaatc
ctgcaccgtgtagtacctcatggccagagctttcaggataattacgctggtgtctttcattttcagctgtggcagtttggcgagtgggtcgatgtggtta
tcgatgattacctccctgttaaagatggaaaacttgtgtttgtgcattcggcttttcgggaatgaattcgggaatgccttgctggaaaaggccctatgcc
cagggcaaatggttgcttaggagggtcctttcctggggaggcggccccttccgaaagggtttttgagggaatttttacccggaaggggggtttaccggaa
gtgggggtttggaaaatggaaaaaaaaagcctcccctaaaagaatttt
3' EST: CF785495 [+]tttttttttttttttcacattacagaatgagcctttatttttggcatctaaaatccagtacaaaatgacactataagagtaactacagatatctgggtca
caggcatgacctaaagaggcatatttgtatccccaaaatacattttggtgacaagaggatagattatctttgcacgctaactacagaggtgagagaagag
cagtctgactggttggtagggtagagaaaggggaatgataggggatgtgagagtgataaaacagataccacgtgggagcagatgcctgagagtgcagata
gagaaatccttatagtattgctttggcaccacagcagcagtagcagcagcagcagcagttgaaaggcactcgcagaaagtcacaggagcgttgggggacc
caacccatgcagaattgggatttttctatgcgaacattgtcatttggagccatttaaaaatgtcaaaatgaatcactcctttcttctctgtgtccatacc
ctggaaaaaacggaacatagtctccagtctgatgagacagcatgtgaagctatcaaaattcacagccatatcaggctctgagtatctggtgatgatgagc
tgatgcaaactgttggttaacttgaaaactgcagactccacagctaaacgcatctcataggcactcatactttccggatttttccctgtcaaaatttcgg
aaaactgtcaagaaatttttaatttttattcccaaaaccatttgaactcccccagtcccaaattttcccattcccatctttatcccaccaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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