XB-CLONE-4350507
Clone Name: IMAGE:7025681
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Gene:
ube2e3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082739 [+]
ATGTTCGGCAGCCGGCCGGAGCCTGCTCGTTTCCCCGGCTCTGCGTTCCCAGGATCCCCCGGCGGCAGCGGCACACACAGCGGCGTGAGGAGAGCAGGGA
CCGGCGCGCCCGGAAGATTGCATCCAGAAGAAACAGAATCCATCAAGATGTCTAGTGATAGACAGCGCTCAGATGATGAAAGCCCTAGTACCAGTAGTGG
AAGTTCAGATGCTGACCAGCGAGACCCACCAGCACCAGAACCTGAAGAGCAAGAAGAAAGAAAACCCTCGGCGGTCCAACAAAAGAAAAATACTAAACTG
TCCAGTAAAACCACTGCTAAGCTGTCCACTAGTGCTAAAAGGATTCAGAAGGAGTTGGCTGAAATAACCCTTGATCCTCCTCCGAACTGCAGCGCTGGAC
CAAAGGGGGACAATATTTATGAATGGAGATCTACGATTCTTGGACCTCCAGGATCAGTATATGAGGGGGGTGTATTTTTCTTGGATATCACATTTTCATC
TGATTATCCCTTTAAGCCACCAAAGGTCACTTTTCGCACCAGAATCTATCACTGTAATATTAACAGCCAGGGAGTAATCTGCTTGGATATTCTGAAAGAT
AACTGGAGTCCAGCATTGACTATATCAAAAGTTTTGCTATCTATTTGTTCCCTCTTGACAGACTGCAATCCCGCTGATCCACTGGTCGGAAGCATAGCCA
CTCAGTACCTGACCAACAGAGCCGAGCATGACAGGATAGCGAGACAGTGGACCAAGAGATATGCTACATAAACTGTACAGTGAAAAGCACAAGGCATCTG
TACAATGTGCAACAAATCTTTATAGCCTTTACAATACGGACTTCTGTGTATATGTTATACTGATTCTACTCTCTGCTTTTATCCTATGAAGACTGGTATA
CTGCCCCAAAAGGTAAATACTAAGAAGAGTAGAACTTTGTAGCTGTAGATTAGTAATGTTAAAATGCCTACTTGCAAGTCTTGCTTCTTTGGGATATCAA
AATGTATTTTGTGATGTACTAAGAATACTGTTCCAAACCAAATATTATAGTGCATTTTAGCCTAATTCATTATCTGTATGAGGTTATTAAAGTTAGCAGT
AGATTACTAGAAATTGTCATTTGTATTAAACCCAGAAATATTTCCAATTATGTGGTAGACACTGTTGTGAAAACTGTTTTACCTTTTGTCAGTTTGAACT
GAAAAGATTTCCTTTATTCCTTTGTAGCTCAGAGAGCACCCAAATGTATTATCCCAAACACACAATAAACATGTTCCCTAAAAAAAAAAAAAAAA
5' EST: CF785482 [+]taattaatcccccccccccccatgttcggcagccggccggagcctgctcgtttccccggctctgcgttcccaggatcccccggcggcagcggcacacaca
gcggcgtgaggagagcagggaccggcgcgcccggaagattgcatccagaagaaacagaatccatcaagatgtctagtgatagacagcgctcagatgatga
aagccctagtaccagtagtggaagttcagatgctgaccagcgagacccaccagcaccagaacctgaagagcaagaagaaagaaaaccctcggcggtccaa
caaaagaaaaatactaaactgtccagtaaaaccactgctaagctgtccactagtgctaaaaggattcagaaggagttggctgaaataacccttgatcctc
ctccgaactgcagcgctggaccaaagggggacaatatttatgaatggagatctacgattcttggacctccaggatcagtatatgaggggggtgtattttt
cttggatatcacattttcatctgattatccctttaagccaccaaaggtcacttttcgcaccagaatctatcactgtaagattaacagccagggagtaatc
tgcttggatattctgaaagataactggagtccagcattgactatatcaaaagtttttgctatctatttgtttccctcttgaacagactgcaatccgcgct
gatcccctgggtcgggaagcatagcccactcagtacctggaccaacaagagcccgagcatggacggggattagcgagaaaagtggggacccaagaaaata
atggctaaccataaaaacctggtaacaggtgga
3' EST: CF785481 [+]tttttttttttttttagggaacatgtttattgtgtgtttgggataatacatttngggtgctctctgagctacaaaggaataaaggaaatcttttcagttc
aaactgacaaaaggtaaaacagttttcacaacagtgtctaccacataattggaaatatttctgggtttaatacaaatgacaatttctagtaatctactgc
taactttaataacctcatacagataatgaattaggctaaaatgcactataatatttggtttggaacagtattcttagtacatcacaaaatacattttgat
atcccaaagaagcaagacttgcaagtaggcattttaacattactaatctacagctacaaagttctactcttcttaaaatttaccttttggggcagtatac
cagtcttcctaggataaaagcagagagtagaatccgtataacatatacccagaagtccgtattgtaaaggctataaagatttgttgcacattggacagat
gcccttggtgctttccctgtacagttttaggtaacatatctcctgggcccactgtctccctatcctggtcagccccggctccgtttggtccgggaacgga
gtggcttatccttccaaacagaggaataccggggattggagtctgttcaaaagggaaacaaatagaatggccaaaacctttttaataaaagtcaaaagct
tgaactcccctttatcttttcaaaaaaatccccaggcaaaataacccccccgtggcagataaaaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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