XB-CLONE-4350513
Clone Name: IMAGE:7025683
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Gene:
mphosph6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077650 [+]
CCGGATTCTCATTCCTGGGACAGCGTGTGAAGGAAGAAGCGAAAGTGGGTAGCGTGTTGCCACGGCTGACACATCATGGCTGCAGAAGTTGGAAAGCTGT
CCAAAAACTTGCTGCGTATGAAGTTTATGCAGCGCACCCTTGATGCAGAGACCAAAAAGCAGTTAGAAGAAGATGAGAAGAAGATAATCAGTGATGAGCA
CTGGTACTTGGATTTACCAGAACTTAAAGAGAAAGAGAGTTATATCATTGAAGAAAGGAGTTTTCTGCCATGTGAAGATTTCCTGTATGGCAGAATGTCC
TTCAAAGGTTTTAACACTGAGGTTGAGAAATTAATGACCCAGCTGAATTCAAGGACAAAGACAGAAGAGATAGTTGAGGAAGGAACAATGGATGTTGATG
TGTCTGATGAAGAAATGGCACGAAGATACGAGTCATTAGTGGGCACCATCAAAAAGAAATTTACGAAAAAAAGGAATCAGAAAAAAAAAAAAAAAA
5' EST: CF785478 [+]taattaatcccccccccccccccggattctcattcctgggacagcgtgtgaaggaagaagcgaaagtgggtagcgtgttgccacggctgacacatcatgg
ctgcagaagttggaaagctgtccaaaaacttgctgcgtatgaagtttatgcagcgcacccttgatgcagagaccaaaaagcagttagaagaagatgagaa
gaagataatcagtgatgagcactggtacttggatttaccagaacttaaagagaaagagagttatatcattgaagaaaggagttttctgccatgtgaagat
ttcctgtatggcagaatgtccttcaaaggttttaacactgaggttgagaaattaatgacccagctgaattcaaggacaaagacagaagagatagttgagg
aaggaacaatggatgttgatgtgtctgatgaagaaatggcacgaagatacgagtcattagtgggcaccatcaaaaagaaatttacgaaaaaaaggaatca
gaaaaaaaaaaaaaaaag
3' EST: CF785477 [+]tttttttttttttttctgattcctttttttcgtaaatttctttttgatggtgcccactaatgactcgtatcttcgtgccatttcttcatcagacacatca
acatccattgttccttcctcaactatctcttctgtctttgtccttgaattcagctgggtcattaatttctcaacctcagtgttaaaacctttgaaggaca
ttctgccatacaggaaatcttcacatggcagaaaactcctttcttcaatgatataactctctttctctttaagttctggtaaatccaagtaccagtgctc
atcactgattatcttcttctcatcttcttctaactgctttttggtctctgcatcaagggtgcgctgcataaacttcatactcagcaagtttttggacagc
tttccaaattctgcagccatgatgtgtcagccgtggcaaaagactacccactttcccttcttccttcaaacgctggtcccaggaaagaaaatcccggggg
ggggggggggaattattaacttcaggggaaaaaaaaaaacaataacctttgatattcgggccccaaaaaaaaccagagttccacattgggacctcgagaa
ttggaaactcctgtggcaataaaaaggcaataggggcaatgttatttttttagtggaccaccaaaactagatttgttatggaaacaaagaaggtcggtca
attaatttgaactccggcatttttaaactgaagcctaaggggggtggggccttaaaaaaacccttttgggaggggatttcaaactactatatctgggcaa
aaggggaaaatcaagcgatttttaagagaaacctgttctcagagcacctgataaagcaaggaatatgtgcacacagaaggcatatctctatgagag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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