XB-CLONE-4350540
Clone Name: IMAGE:7025697
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Gene:
vbp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077711 [+]
ACTGCTTCCAAGATGGCGGTGACCAGTGAGGGAGCCGGTGTTGCTGGACTGAAACGGACTCATTTGGGAATTCCGGAGGCAGTGTTTGTGGAAGATGTAG
ATGCTTTTATGAAGAAACCTGGAAATGAGACAGCAGATGCAGTACTCAAGAAACTGGATGAGCAGTATCAGAAATATAAATTTATGGAACTTAATTTGAC
CCAGAAAAAAAGAAGGCTAAAGAGCCAGATTCCAGAAATTAAGCAGACCTTAGAAATTTTAAAACACATGCAGAAGAAAAAGGATACTACTGAACCAATG
AAAACTAGGTTTCTGCTAGCCGATAATCTGTACTGTAAAGCATCTGTGCCACCTACAGACAAAGTCTGCCTGTGGTTGGGGGCAAATGTTATGCTTGAGT
ACGATATTGATGAAGCTCAGGCTTTACTTGAAAAGAATCTTTCAACTGCTACAAGAAACCTTGATTCTACAGAAGAAGACCTCGACTTCCTTAGGGACCA
GTTCACTACTACAGAAGTCAATATGGCTAGAGTTTATAATTGGGATGCAAAAAAAAAAAAAAAA
5' EST: CF785461 [+]taattaatccccccccccccgactgcttccaagatggcggtgaccagtgagggagccggtgttgctggactgaaacggactcatttgggaattccggagg
cagtgtttgtggaagatgtagatgcttttatgaagaaacctggaaatgagacagcagatgcagtactcaagaaactggatgagcagtatcagaaatataa
atttatggaacttaatttgacccagaaaaaaagaaggctaaagagccagattccagaaattaagcagaccttagaaattttaaaacacatgcagaagaaa
aaggatactactgaaccaatgaaaactaggtttctgctagccgataatctgtactgtaaagcatctgtgccacctacagacaaagtctgcctgtggttgg
gggcaaatgttatgcttgagtacgatattgatgaagctcaggctttacttgaaaagaatctttcaactgctacaagaaaccttgattctacagaagaaga
cctcgacttccttagggaccagttcactactacagaagtcaatatggctagagtttataattgggatgcaaaaaaaaaaaaaaaag
3' EST: CF785460 [+]tttttttttttttttgcatcccaattataaactctagccatattgacttctgtagtagtgaactggtccctaaggaagtcgaggtcttcttctgtagaat
caaggtttcttgtagcagttgaaagattcttttcaagtaaagcctgagcttcatcaatatcgtactcaagcataacatttgcccccaaccacaggcagac
tttgtctgtaggtggcacagatgctttacagtacagattatcggctagcagaaacctagttttcattggttcagtagtatcctttttcttctgcatgtgt
tttaaaatttctaaggtctgcttaatttctggaatctggctctttagccttctttttttctgggtcaaattaagttccataaatttatatttctgatact
gctcatccagtttcttgagtactgcatctgctgtctcatttccaggtttcttcataaaagcatctacatcttccacaaacactgcctccggaattcccaa
atgagtccgtttcagtccagcaacaccggctccctcactggtcaccgccatcttggaagcagtcggggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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