XB-CLONE-4350543
Clone Name: IMAGE:7025698
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Gene:
h3-3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785458 [+]
taattaatcccccccccccccgacgttagtttgtctcctcgtttgaccgtttgggttacgctcccgggagtgatcgaataacaagaagcaatggctcgta
caaagcaaactgcccgtaaatccactggtggaaaggctccaaggaagcagctggctacaaaggctgccaggaaaagtgctccttccacaggtggtgtgaa
gaagccacacagatacaggcctggcactgttgccttgagagaaatcagaagatatcagaagtctactgagctgttgatccgaaaactaccattccagcga
cttgtaagggagattgcccaagacttcaagactgatttgaggttccagagtgcagctattggtgctcttcaggaagcaagtgaggcttacctggttggcc
tctttgaagataccaacctgtgtgccattcatgccaagagagtaacaattatgcccaaggacatccagttagcaagaagaatccgtggcgagcgtgctta
aacattttgctaggaacttttttttgaacttagttttttttttacaattgtacatacaagtgtgatgtcttttattttataaagggtttgttaactgtag
agtagttgacagtaagatggtagtaaacattttatatgacattcccttaatcctcaggtttttcagaaatttgtatctgcagctagtcttttgtgaccct
ctcctttaaacataatgcatgcccatacttcattctttacataatttagtatctttctgtgtaacttccttgg
3' EST: CF785459 [+]tttttttttttttttttttttttttttttttttttttttttgagggaggggaaagtttatttacaatggagttacccagaaagatactaaattatgtaaa
gaatgaagtatgggcatgcactatgttttaaaggagagggtcacaaaagactagctgcagatacaaatttctgaaaaacctgaggattaagggaatgtcc
tataaaatgtttactaccatcttactgtcaactactctacagttaacaaaccctttataaaataaaagacatcacacttgtatgtacaattgtaaaaaaa
aaactaagttcaaaaaaaagttcctagcaaaatgtttaagcacgctcgccacggattcttcttgctaactggatgtccttgggcataattgttactctct
tggcatgaatggcacacaggttggtatcttcaaagaggccaaccaggtaagcctcacttgcttcctgaagagcaccaatagctgcactctggaacctcaa
atcagtcttgaagtcttgggcaatctcccttacaagtcgctgggatggtagttttcggatcaacagctcagtagacttctgatatcttctgatttcctca
aggcaacagtgccaggcctgtatctgtgtgggcttctcacaccactgtggaaagagcacttttctggcagccttttgtaccagctgcttcccttggagcc
tttccaccaaggggatttacgggcaggtttgcctttgtacgaacccattgcttcttgtttattcgatccactcccgggggagcgtaaaccaaaacgggtc
aaacgagggagacaaactaacgtccgggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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